Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890199835> ?p ?o ?g. }
- W2890199835 abstract "Biosynthesis of steroidal drugs is of great benefit in pharmaceutical manufacturing as the process involves efficient enzymatic catalysis at ambient temperature and atmospheric pressure compared to chemical synthesis. 3-ketosteroid-∆1-dehydrogenase from Arthrobacter simplex (KsdD3) catalyzes 1,2-desaturation of steroidal substrates with FAD as a cofactor.Recombinant KsdD3 exhibited organic solvent tolerance. W117, F296, W299, et al., which were located in substrate-binding cavity, were predicted to form hydrophobic interaction with the substrate. Structure-based site-directed saturation mutagenesis of KsdD3 was performed with W299 mutants, which resulted in improved catalytic activities toward various steroidal substrates. W299A showed the highest increase in catalytic efficiency (kcat/Km) compared with the wild-type enzyme. Homology modelling revealed that the mutants enlarged the active site cavity and relieved the steric interference facilitating recognition of C17 hydroxyl/carbonyl steroidal substrates. Steered molecular dynamics simulations revealed that W299A/G decreased the potential energy barrier of association of substrates and dissociation of the corresponding products. The biotransformation of AD with enzymatic catalysis and resting cells harbouring KsdD3 WT/mutants revealed that W299A catalyzed the maximum ADD yields of 71 and 95% by enzymatic catalysis and resting cell conversion respectively, compared with the wild type (38 and 75%, respectively).The successful rational design of functional KsdD3 greatly advanced our understanding of KsdD family enzymes. Structure-based site-directed saturation mutagenesis and biochemical data were used to design KsdD3 mutants with a higher catalytic activity and broader selectivity." @default.
- W2890199835 created "2018-09-27" @default.
- W2890199835 creator A5005637556 @default.
- W2890199835 creator A5012613471 @default.
- W2890199835 creator A5017114379 @default.
- W2890199835 creator A5026266621 @default.
- W2890199835 creator A5031084065 @default.
- W2890199835 creator A5031141261 @default.
- W2890199835 creator A5040547672 @default.
- W2890199835 creator A5057289300 @default.
- W2890199835 creator A5060741317 @default.
- W2890199835 creator A5071861102 @default.
- W2890199835 creator A5081391258 @default.
- W2890199835 creator A5081978498 @default.
- W2890199835 creator A5090254078 @default.
- W2890199835 date "2018-09-10" @default.
- W2890199835 modified "2023-10-17" @default.
- W2890199835 title "Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates" @default.
- W2890199835 cites W1031578623 @default.
- W2890199835 cites W126943371 @default.
- W2890199835 cites W1845918780 @default.
- W2890199835 cites W193860077 @default.
- W2890199835 cites W1972675724 @default.
- W2890199835 cites W1975201705 @default.
- W2890199835 cites W1984367711 @default.
- W2890199835 cites W1985328407 @default.
- W2890199835 cites W1987260214 @default.
- W2890199835 cites W1987304610 @default.
- W2890199835 cites W1988193571 @default.
- W2890199835 cites W1990951667 @default.
- W2890199835 cites W2010355774 @default.
- W2890199835 cites W2012130346 @default.
- W2890199835 cites W2016490429 @default.
- W2890199835 cites W2017114583 @default.
- W2890199835 cites W2026177688 @default.
- W2890199835 cites W2044740604 @default.
- W2890199835 cites W2046688405 @default.
- W2890199835 cites W2057708531 @default.
- W2890199835 cites W2059438873 @default.
- W2890199835 cites W2059875409 @default.
- W2890199835 cites W2065283382 @default.
- W2890199835 cites W2070372139 @default.
- W2890199835 cites W2075781418 @default.
- W2890199835 cites W2076264079 @default.
- W2890199835 cites W2082806783 @default.
- W2890199835 cites W2089016273 @default.
- W2890199835 cites W2110565490 @default.
- W2890199835 cites W2131777168 @default.
- W2890199835 cites W2132236615 @default.
- W2890199835 cites W2137068535 @default.
- W2890199835 cites W2154935175 @default.
- W2890199835 cites W2158363360 @default.
- W2890199835 cites W2283144829 @default.
- W2890199835 cites W2312837368 @default.
- W2890199835 cites W2564181803 @default.
- W2890199835 cites W2593644429 @default.
- W2890199835 cites W2604459153 @default.
- W2890199835 cites W2668500951 @default.
- W2890199835 cites W2680282270 @default.
- W2890199835 cites W2734409275 @default.
- W2890199835 cites W2755636212 @default.
- W2890199835 cites W2770902816 @default.
- W2890199835 cites W2781615191 @default.
- W2890199835 cites W4230572783 @default.
- W2890199835 doi "https://doi.org/10.1186/s12934-018-0981-0" @default.
- W2890199835 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6130075" @default.
- W2890199835 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30200975" @default.
- W2890199835 hasPublicationYear "2018" @default.
- W2890199835 type Work @default.
- W2890199835 sameAs 2890199835 @default.
- W2890199835 citedByCount "16" @default.
- W2890199835 countsByYear W28901998352019 @default.
- W2890199835 countsByYear W28901998352020 @default.
- W2890199835 countsByYear W28901998352021 @default.
- W2890199835 countsByYear W28901998352022 @default.
- W2890199835 countsByYear W28901998352023 @default.
- W2890199835 crossrefType "journal-article" @default.
- W2890199835 hasAuthorship W2890199835A5005637556 @default.
- W2890199835 hasAuthorship W2890199835A5012613471 @default.
- W2890199835 hasAuthorship W2890199835A5017114379 @default.
- W2890199835 hasAuthorship W2890199835A5026266621 @default.
- W2890199835 hasAuthorship W2890199835A5031084065 @default.
- W2890199835 hasAuthorship W2890199835A5031141261 @default.
- W2890199835 hasAuthorship W2890199835A5040547672 @default.
- W2890199835 hasAuthorship W2890199835A5057289300 @default.
- W2890199835 hasAuthorship W2890199835A5060741317 @default.
- W2890199835 hasAuthorship W2890199835A5071861102 @default.
- W2890199835 hasAuthorship W2890199835A5081391258 @default.
- W2890199835 hasAuthorship W2890199835A5081978498 @default.
- W2890199835 hasAuthorship W2890199835A5090254078 @default.
- W2890199835 hasBestOaLocation W28901998351 @default.
- W2890199835 hasConcept C103408237 @default.
- W2890199835 hasConcept C104317684 @default.
- W2890199835 hasConcept C109129352 @default.
- W2890199835 hasConcept C143065580 @default.
- W2890199835 hasConcept C147816474 @default.
- W2890199835 hasConcept C161790260 @default.
- W2890199835 hasConcept C16318435 @default.
- W2890199835 hasConcept C181199279 @default.
- W2890199835 hasConcept C185592680 @default.