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• W2890202370 abstract "White spot syndrome virus (WSSV) has a ∼300 kb double-stranded DNA genome. It originated in China, spread rapidly through shrimp farms in Asia, and subsequently to America. This study determined complete genome sequences for nine historic WSSV strains isolated from Pacific white shrimp (Litopenaeus vannamei) captured in farm ponds in northwest Mexico (Sinaloa and Nayarit). Genomic DNA was captured by an amplification method using overlapping long-range PCR and sequencing by Ion Torrent-PGM. Complete genome sequences were assembled (length range 255-290 kb) and comparative genome analysis with WSSV strains revealed substantial deletions (3 and 10 kb in two regions) in seven strains, with two strains differing from the rest. Phylogenetic analysis identified that the WSSV strains from the northern area of the state of Sinaloa clustered with strains from China (LC1, LC10, DVI) and Korea (ACF2, ACF4), while those from the southern region of the state of Nayarit (AC1 and JP) differed from both of those and from strains found in Taiwan and Thailand. Our data offer insights into the diversity of the WSSV genome in one country and their divergent origin, suggest that it entered Mexico via multiple routes and that specific genome regions can accommodate substantial deletions without compromising viability." @default.
• W2890202370 created "2018-09-27" @default.
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• W2890202370 date "2018-09-04" @default.
• W2890202370 modified "2023-10-16" @default.
• W2890202370 title "Molecular variability and genetic structure of white spot syndrome virus strains from northwest Mexico based on the analysis of genomes" @default.
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• W2890202370 doi "https://doi.org/10.1093/femsle/fny216" @default.
• W2890202370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30184198" @default.
• W2890202370 hasPublicationYear "2018" @default.
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