Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890219944> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2890219944 abstract "Multiple mechanisms regulate aromatic compound degradation in the soil bacterium Acinetobacter baylyi ADP1. Herein, complementary mechanisms controlling the consumption of benzoate are explored: 1) transcriptional regulation and 2) chromosomal amplification of catabolic genes. The former highlights BenM, a member of the LysR family of regulators controlling transcription of a complex regulon for benzoate degradation in ADP1. BenM activates gene expression in response to two effectors, benzoate and muconate. These effectors act synergistically to allow high-level gene expression, a feature not characterized in any other member of this broad family. Here, the unique effector profile of BenM is explored. Genetic studies, combined with structural work, elucidate the role of two distinct effector-binding sites in BenM. Targeted mutagenesis confirms the physiological relevance of a hydrophobic benzoate binding pocket and pinpoints critical residues, Arg160 and Tyr293, essential for benzoate-dependent gene expression and synergistic transcriptional activation. BenM variants that activate transcription in the absence of inducer are examined: BenM(R156H), BenM(R225H), and BenM(E226K). Structural studies of BenM variants reveal subtle conformational changes associated with transcriptional activation.The recent discovery of gene amplification in ADP1 highlights an alternate strategy of genetic regulation. In strains lacking wild-type regulation, chromosomal amplification of catabolic genes can compensate for low transcript levels. Here, a wide spectrum of amplification events were studied using multiple approaches uniquely available in the tractable ADP1 model system. Approximately 50 amplification mutants with enhanced benzoate catabolism were characterized. Each harbors multiple copies of a genomic region, ranging from 12 kbp to more than 300 kbp, encompassing the ben and cat functional genes. Analysis of the precise sequence at amplicon endpoints identifies the DNA sequence involved in formation of the original duplication. In all cases examined, these sequences indicate genetic duplication was initiated by a homology-independent illegitimate recombination mechanism. Recurrence of exact duplication junctions in multiple mutants reveals site preference in duplication formation, a mechanism we designate position-specific illegitimate recombination. The novel experimental tools developed here will facilitate a rapid and thorough examination of the full spectrum of gene amplification events contributing to ADP1 genome plasticity, as well as enable studies into the relevant recombination mechanisms." @default.
- W2890219944 created "2018-09-27" @default.
- W2890219944 creator A5088410730 @default.
- W2890219944 date "2009-01-01" @default.
- W2890219944 modified "2023-09-23" @default.
- W2890219944 title "Gene expression and dosage: distinct mechanisms regulate benzoate degradation in Acinetobacter baylyi ADP1" @default.
- W2890219944 hasPublicationYear "2009" @default.
- W2890219944 type Work @default.
- W2890219944 sameAs 2890219944 @default.
- W2890219944 citedByCount "0" @default.
- W2890219944 crossrefType "journal-article" @default.
- W2890219944 hasAuthorship W2890219944A5088410730 @default.
- W2890219944 hasConcept C104317684 @default.
- W2890219944 hasConcept C138885662 @default.
- W2890219944 hasConcept C143065580 @default.
- W2890219944 hasConcept C150194340 @default.
- W2890219944 hasConcept C158448853 @default.
- W2890219944 hasConcept C161733203 @default.
- W2890219944 hasConcept C165864922 @default.
- W2890219944 hasConcept C179926584 @default.
- W2890219944 hasConcept C27153228 @default.
- W2890219944 hasConcept C41895202 @default.
- W2890219944 hasConcept C51785407 @default.
- W2890219944 hasConcept C54355233 @default.
- W2890219944 hasConcept C55493867 @default.
- W2890219944 hasConcept C62361671 @default.
- W2890219944 hasConcept C7602840 @default.
- W2890219944 hasConcept C86803240 @default.
- W2890219944 hasConceptScore W2890219944C104317684 @default.
- W2890219944 hasConceptScore W2890219944C138885662 @default.
- W2890219944 hasConceptScore W2890219944C143065580 @default.
- W2890219944 hasConceptScore W2890219944C150194340 @default.
- W2890219944 hasConceptScore W2890219944C158448853 @default.
- W2890219944 hasConceptScore W2890219944C161733203 @default.
- W2890219944 hasConceptScore W2890219944C165864922 @default.
- W2890219944 hasConceptScore W2890219944C179926584 @default.
- W2890219944 hasConceptScore W2890219944C27153228 @default.
- W2890219944 hasConceptScore W2890219944C41895202 @default.
- W2890219944 hasConceptScore W2890219944C51785407 @default.
- W2890219944 hasConceptScore W2890219944C54355233 @default.
- W2890219944 hasConceptScore W2890219944C55493867 @default.
- W2890219944 hasConceptScore W2890219944C62361671 @default.
- W2890219944 hasConceptScore W2890219944C7602840 @default.
- W2890219944 hasConceptScore W2890219944C86803240 @default.
- W2890219944 hasLocation W28902199441 @default.
- W2890219944 hasOpenAccess W2890219944 @default.
- W2890219944 hasPrimaryLocation W28902199441 @default.
- W2890219944 hasRelatedWork W1492615693 @default.
- W2890219944 hasRelatedWork W1578805890 @default.
- W2890219944 hasRelatedWork W1824400675 @default.
- W2890219944 hasRelatedWork W1985674058 @default.
- W2890219944 hasRelatedWork W1986598634 @default.
- W2890219944 hasRelatedWork W2001257630 @default.
- W2890219944 hasRelatedWork W2023105172 @default.
- W2890219944 hasRelatedWork W2042075518 @default.
- W2890219944 hasRelatedWork W2047696813 @default.
- W2890219944 hasRelatedWork W2056491244 @default.
- W2890219944 hasRelatedWork W2129770809 @default.
- W2890219944 hasRelatedWork W2143462323 @default.
- W2890219944 hasRelatedWork W2153018631 @default.
- W2890219944 hasRelatedWork W2154223238 @default.
- W2890219944 hasRelatedWork W2157084647 @default.
- W2890219944 hasRelatedWork W2204964609 @default.
- W2890219944 hasRelatedWork W2402707285 @default.
- W2890219944 hasRelatedWork W2800814226 @default.
- W2890219944 hasRelatedWork W2965357619 @default.
- W2890219944 hasRelatedWork W3096744646 @default.
- W2890219944 isParatext "false" @default.
- W2890219944 isRetracted "false" @default.
- W2890219944 magId "2890219944" @default.
- W2890219944 workType "article" @default.