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• W2890260657 abstract "cccDNA elimination is a major goal in future curative regimens for chronic HBV patients. However, PCR-based assays for cccDNA quantification show a principally constrained specificity when high levels of input virus or replicative intermediates are present. Here, we characterized T5 exonuclease as a suitable enzyme for medium-throughput in vitro assays that preserves cccDNA but efficiently removes rcDNA prior to PCR-based quantification. We compared T5 exonuclease with the previously described exonuclease III and showed that both nucleases are suitable for reliable quantification of cccDNA by PCR. We substantiated the applicability of our method through examination of early cccDNA formation and stable accumulation in several in vitro infection models and analyzed cccDNA stability after administration of anti-HBV drugs. Our results support the use of T5 exonuclease for fast and convenient rcDNA removal, especially for early cccDNA quantification and rapid drug testing in in vitro studies." @default.
• W2890260657 created "2018-09-27" @default.
• W2890260657 creator A5009012266 @default.
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• W2890260657 date "2018-12-01" @default.
• W2890260657 modified "2023-10-16" @default.
• W2890260657 title "T5 Exonuclease Hydrolysis of Hepatitis B Virus Replicative Intermediates Allows Reliable Quantification and Fast Drug Efficacy Testing of Covalently Closed Circular DNA by PCR" @default.
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• W2890260657 doi "https://doi.org/10.1128/jvi.01117-18" @default.
• W2890260657 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6232461" @default.
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• W2890260657 hasPublicationYear "2018" @default.
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