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- W2890351601 abstract "Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent. This chapter provides examples for the production and purification of a his-tagged recombinant protein and two monoclonal antibodies." @default.
- W2890351601 created "2018-09-27" @default.
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- W2890351601 date "2018-01-01" @default.
- W2890351601 modified "2023-09-25" @default.
- W2890351601 title "Transient Gene Expression in Suspension HEK293-EBNA1 Cells" @default.
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- W2890351601 doi "https://doi.org/10.1007/978-1-4939-8730-6_1" @default.
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