FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890375249> ?p ?o ?g. }

• W2890375249 abstract "ABSTRACT Malaria is a debilitating parasitic disease that causes significant morbidity and mortality. Microscopic detection of parasites is currently the “gold standard” diagnostic. This technique is limited in its ability to detect low-density infections, is time consuming, and requires a highly trained microscopist. Malaria epidemiological surveillance studies especially aimed at the detection of low-density infection and asymptomatic cases will require more sensitive and user-friendly tools. We have shown previously that the molecular-based, colorimetric malachite green loop-mediated isothermal amplification (MG-LAMP) assay is a valuable tool for diagnosing malaria infection in a laboratory setting. In this study, we field evaluated this assay in a malaria diagnostic post in Roraima, Brazil. We prospectively collected 91 patient samples and performed microscopy, MG-LAMP, and real-time PCR (PET-PCR) to detect Plasmodium infection. Two independent readers were used to score the MG-LAMP tests to assess whether the sample was positive (blue/green) or negative (clear). There was 100% agreement between the two readers (Kappa=1). All tests detected 33 positive samples, but both the MG-LAMP and PET-PCR detected 6 and 7 more positive samples, respectively. The PET-PCR assay detected 6 mixed infections (defined as infection with both P. falciparum and P. vivax ) while microscopy detected one and MG-LAMP detected two of these mixed infections. Microscopy did not detect any Plasmodium infection in 26 of the enrolled asymptomatic cases while MG-LAMP detected five and PET-PCR assay three positive cases. Overall, MG-LAMP provided a simpler and user-friendly molecular method for malaria diagnosis that is more sensitive than microscopy. Additionally, MG- LAMP has the capacity to test 38 samples per run (one hour), allowing for the screening of large number of samples which is appealing when large-scale studies are necessary e.g. in community surveillance studies. The current MG-LAMP assay was limited in its ability to detect mixed infection when compared to the PET-PCR, but otherwise proved to be a powerful tool for malaria parasite detection in the field and opens new perspectives in the implementation of surveillance studies in malaria elimination campaigns." @default.
• W2890375249 created "2018-09-27" @default.
• W2890375249 creator A5002337879 @default.
• W2890375249 creator A5024881399 @default.
• W2890375249 creator A5054964707 @default.
• W2890375249 creator A5070332549 @default.
• W2890375249 creator A5071135327 @default.
• W2890375249 creator A5078009221 @default.
• W2890375249 creator A5088443573 @default.
• W2890375249 date "2018-09-05" @default.
• W2890375249 modified "2023-10-14" @default.
• W2890375249 title "Field evaluation of malachite green loop-mediated isothermal amplification as a malaria parasite detection tool in a health post in Roraima state, Brazil" @default.
• W2890375249 cites W1888099230 @default.
• W2890375249 cites W1991198490 @default.
• W2890375249 cites W2010328983 @default.
• W2890375249 cites W2041149170 @default.
• W2890375249 cites W2080499055 @default.
• W2890375249 cites W2081875665 @default.
• W2890375249 cites W2115837962 @default.
• W2890375249 cites W2122562099 @default.
• W2890375249 cites W2126772692 @default.
• W2890375249 cites W2127560679 @default.
• W2890375249 cites W2138505818 @default.
• W2890375249 cites W2143917555 @default.
• W2890375249 cites W2144345536 @default.
• W2890375249 cites W2152873423 @default.
• W2890375249 cites W2284125637 @default.
• W2890375249 cites W2294591031 @default.
• W2890375249 cites W2294929393 @default.
• W2890375249 cites W2399484661 @default.
• W2890375249 cites W2414781386 @default.
• W2890375249 cites W2511561561 @default.
• W2890375249 cites W2550670300 @default.
• W2890375249 cites W2603458750 @default.
• W2890375249 cites W2676940784 @default.
• W2890375249 doi "https://doi.org/10.1101/408609" @default.
• W2890375249 hasPublicationYear "2018" @default.
• W2890375249 type Work @default.
• W2890375249 sameAs 2890375249 @default.
• W2890375249 citedByCount "0" @default.
• W2890375249 crossrefType "posted-content" @default.
• W2890375249 hasAuthorship W2890375249A5002337879 @default.
• W2890375249 hasAuthorship W2890375249A5024881399 @default.
• W2890375249 hasAuthorship W2890375249A5054964707 @default.
• W2890375249 hasAuthorship W2890375249A5070332549 @default.
• W2890375249 hasAuthorship W2890375249A5071135327 @default.
• W2890375249 hasAuthorship W2890375249A5078009221 @default.
• W2890375249 hasAuthorship W2890375249A5088443573 @default.
• W2890375249 hasBestOaLocation W28903752491 @default.
• W2890375249 hasConcept C126322002 @default.
• W2890375249 hasConcept C136764020 @default.
• W2890375249 hasConcept C142724271 @default.
• W2890375249 hasConcept C150394285 @default.
• W2890375249 hasConcept C159047783 @default.
• W2890375249 hasConcept C178790620 @default.
• W2890375249 hasConcept C185592680 @default.
• W2890375249 hasConcept C203014093 @default.
• W2890375249 hasConcept C2777910003 @default.
• W2890375249 hasConcept C2778048844 @default.
• W2890375249 hasConcept C2778371730 @default.
• W2890375249 hasConcept C2779997623 @default.
• W2890375249 hasConcept C2780704308 @default.
• W2890375249 hasConcept C2909011893 @default.
• W2890375249 hasConcept C40993552 @default.
• W2890375249 hasConcept C41008148 @default.
• W2890375249 hasConcept C54355233 @default.
• W2890375249 hasConcept C552990157 @default.
• W2890375249 hasConcept C60635243 @default.
• W2890375249 hasConcept C71924100 @default.
• W2890375249 hasConcept C71928629 @default.
• W2890375249 hasConcept C86803240 @default.
• W2890375249 hasConceptScore W2890375249C126322002 @default.
• W2890375249 hasConceptScore W2890375249C136764020 @default.
• W2890375249 hasConceptScore W2890375249C142724271 @default.
• W2890375249 hasConceptScore W2890375249C150394285 @default.
• W2890375249 hasConceptScore W2890375249C159047783 @default.
• W2890375249 hasConceptScore W2890375249C178790620 @default.
• W2890375249 hasConceptScore W2890375249C185592680 @default.
• W2890375249 hasConceptScore W2890375249C203014093 @default.
• W2890375249 hasConceptScore W2890375249C2777910003 @default.
• W2890375249 hasConceptScore W2890375249C2778048844 @default.
• W2890375249 hasConceptScore W2890375249C2778371730 @default.
• W2890375249 hasConceptScore W2890375249C2779997623 @default.
• W2890375249 hasConceptScore W2890375249C2780704308 @default.
• W2890375249 hasConceptScore W2890375249C2909011893 @default.
• W2890375249 hasConceptScore W2890375249C40993552 @default.
• W2890375249 hasConceptScore W2890375249C41008148 @default.
• W2890375249 hasConceptScore W2890375249C54355233 @default.
• W2890375249 hasConceptScore W2890375249C552990157 @default.
• W2890375249 hasConceptScore W2890375249C60635243 @default.
• W2890375249 hasConceptScore W2890375249C71924100 @default.
• W2890375249 hasConceptScore W2890375249C71928629 @default.
• W2890375249 hasConceptScore W2890375249C86803240 @default.
• W2890375249 hasLocation W28903752491 @default.
• W2890375249 hasOpenAccess W2890375249 @default.
• W2890375249 hasPrimaryLocation W28903752491 @default.
• W2890375249 hasRelatedWork W1854497137 @default.
• W2890375249 hasRelatedWork W1989196734 @default.
• W2890375249 hasRelatedWork W2059582865 @default.
• W2890375249 hasRelatedWork W2144827649 @default.

• next