Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890425074> ?p ?o ?g. }
- W2890425074 abstract "Fibroblast behavior and cell-matrix interactions of cells from normal and idiopathic carpal tunnel syndrome (CTS) subsynovial connective tissue (SSCT) with and without Triamcinolone Acetonide (TA) were compared in this study. A cell-seeded gel contraction model was applied to investigate the effect of steroid treatment on SSCT fibroblast gene expression and function.SSCT cells were obtained from CTS patients and fresh cadavers. Cells were isolated by mechanical and collagenase digestion. Collagen gels (1 mg/ml) were prepared with SSCT cells (1 × 106/mL). A sterile Petri dish with a cloning ring in the center was prepared. The area between the ring and outer dish was filled with cell-seeded collagen solution and gelled for 1 h. The gel was released from the outer way of the petri dish to allow gel contraction. Cell seeded gels were treated with 10 M triamcinolone acetonide (TA) or vehicle (DMSO) in modified MEM. Every 4 h for 3 days the contracting gels were photographed and areas calculated. Duplicate contraction tests were performed with each specimen, and the averages were used in the analyses, which were conducted using two-factor analysis of variance in a generalized linear model framework utilizing generalized estimating equations (GEE) to account for the correlation between samples. The contraction rate was determined by the area change over time, and the decay time constant was calculated. A customized mechanical test system was used to determine gel stiffness and tensile strength. Gene expression was assessed using Human Fibrosis and Cell Motility PCR arrays.TA-treated gels had a significantly higher contraction rate, tensile strength and stiffness than the untreated gels. Proteinases involved in remodeling had increased expression in TA-treated gels of the patient group. Pro-fibrotic genes and ECM regulators, such as TGF-β, collagens and integrins, were down-regulated by TA, indicating that TA may work in part by decreasing fibrotic gene expression.This study showed that TA affects cell-matrix interaction and suppresses fibrotic gene expression in the SSCT cells of CTS patients." @default.
- W2890425074 created "2018-09-27" @default.
- W2890425074 creator A5007359838 @default.
- W2890425074 creator A5011690849 @default.
- W2890425074 creator A5046286865 @default.
- W2890425074 creator A5071863751 @default.
- W2890425074 creator A5075508225 @default.
- W2890425074 creator A5083191298 @default.
- W2890425074 date "2018-09-22" @default.
- W2890425074 modified "2023-10-13" @default.
- W2890425074 title "Triamcinolone Acetonide affects TGF-β signaling regulation of fibrosis in idiopathic carpal tunnel syndrome" @default.
- W2890425074 cites W1494758078 @default.
- W2890425074 cites W1564067955 @default.
- W2890425074 cites W184982434 @default.
- W2890425074 cites W1968721537 @default.
- W2890425074 cites W1969128948 @default.
- W2890425074 cites W1972978962 @default.
- W2890425074 cites W1973719115 @default.
- W2890425074 cites W1983714352 @default.
- W2890425074 cites W1984729535 @default.
- W2890425074 cites W1986490376 @default.
- W2890425074 cites W1989363185 @default.
- W2890425074 cites W1990932340 @default.
- W2890425074 cites W2010149891 @default.
- W2890425074 cites W2015216824 @default.
- W2890425074 cites W2027585163 @default.
- W2890425074 cites W2027875827 @default.
- W2890425074 cites W2031029504 @default.
- W2890425074 cites W2062262109 @default.
- W2890425074 cites W2062954025 @default.
- W2890425074 cites W2068125278 @default.
- W2890425074 cites W2071913015 @default.
- W2890425074 cites W2090169675 @default.
- W2890425074 cites W2099722910 @default.
- W2890425074 cites W2109999257 @default.
- W2890425074 cites W2112195516 @default.
- W2890425074 cites W2112483353 @default.
- W2890425074 cites W2115044953 @default.
- W2890425074 cites W2127469660 @default.
- W2890425074 cites W2137842351 @default.
- W2890425074 cites W2151490263 @default.
- W2890425074 cites W2153800430 @default.
- W2890425074 cites W2273184526 @default.
- W2890425074 cites W2319097128 @default.
- W2890425074 cites W2322126443 @default.
- W2890425074 cites W2331046177 @default.
- W2890425074 cites W2432462841 @default.
- W2890425074 cites W4319688102 @default.
- W2890425074 cites W69686190 @default.
- W2890425074 doi "https://doi.org/10.1186/s12891-018-2260-y" @default.
- W2890425074 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6151186" @default.
- W2890425074 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30243295" @default.
- W2890425074 hasPublicationYear "2018" @default.
- W2890425074 type Work @default.
- W2890425074 sameAs 2890425074 @default.
- W2890425074 citedByCount "10" @default.
- W2890425074 countsByYear W28904250742021 @default.
- W2890425074 countsByYear W28904250742022 @default.
- W2890425074 countsByYear W28904250742023 @default.
- W2890425074 crossrefType "journal-article" @default.
- W2890425074 hasAuthorship W2890425074A5007359838 @default.
- W2890425074 hasAuthorship W2890425074A5011690849 @default.
- W2890425074 hasAuthorship W2890425074A5046286865 @default.
- W2890425074 hasAuthorship W2890425074A5071863751 @default.
- W2890425074 hasAuthorship W2890425074A5075508225 @default.
- W2890425074 hasAuthorship W2890425074A5083191298 @default.
- W2890425074 hasBestOaLocation W28904250741 @default.
- W2890425074 hasConcept C105702510 @default.
- W2890425074 hasConcept C134018914 @default.
- W2890425074 hasConcept C141071460 @default.
- W2890425074 hasConcept C142724271 @default.
- W2890425074 hasConcept C146763376 @default.
- W2890425074 hasConcept C163415756 @default.
- W2890425074 hasConcept C181199279 @default.
- W2890425074 hasConcept C185592680 @default.
- W2890425074 hasConcept C189165786 @default.
- W2890425074 hasConcept C202751555 @default.
- W2890425074 hasConcept C2778394429 @default.
- W2890425074 hasConcept C2778903070 @default.
- W2890425074 hasConcept C2779746960 @default.
- W2890425074 hasConcept C2780381497 @default.
- W2890425074 hasConcept C2780559512 @default.
- W2890425074 hasConcept C518705261 @default.
- W2890425074 hasConcept C54355233 @default.
- W2890425074 hasConcept C55493867 @default.
- W2890425074 hasConcept C71924100 @default.
- W2890425074 hasConcept C86803240 @default.
- W2890425074 hasConceptScore W2890425074C105702510 @default.
- W2890425074 hasConceptScore W2890425074C134018914 @default.
- W2890425074 hasConceptScore W2890425074C141071460 @default.
- W2890425074 hasConceptScore W2890425074C142724271 @default.
- W2890425074 hasConceptScore W2890425074C146763376 @default.
- W2890425074 hasConceptScore W2890425074C163415756 @default.
- W2890425074 hasConceptScore W2890425074C181199279 @default.
- W2890425074 hasConceptScore W2890425074C185592680 @default.
- W2890425074 hasConceptScore W2890425074C189165786 @default.
- W2890425074 hasConceptScore W2890425074C202751555 @default.
- W2890425074 hasConceptScore W2890425074C2778394429 @default.
- W2890425074 hasConceptScore W2890425074C2778903070 @default.
- W2890425074 hasConceptScore W2890425074C2779746960 @default.