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• W2890427044 endingPage "e12952" @default.
• W2890427044 startingPage "e12952" @default.
• W2890427044 abstract "PE_PGRSs of Mycobacterium tuberculosis (Mtb) represent a family of complex and peculiar proteins whose role and function remain elusive. In this study, we investigated PE_PGRS3 and PE_PGRS4, two highly homologous PE_PGRSs encoded by two contiguous genes in the Mtb genome. Using a gene-reporter system in Mycobacterium smegmatis (Ms) and transcriptional analysis in Mtb, we show that PE_PGRS3, but not PE_PGRS4, is specifically expressed under low phosphate concentrations. Interestingly, PE_PGRS3, but not PE_PGRS4, has a unique, arginine-rich C-terminal domain of unknown function. Heterologous expression of PE_PGRS3 in Ms was used to demonstrate cellular localisation of the protein on the mycobacterial surface, where it significantly affects net surface charge. Moreover, expression of full-length PE_PGRS3 enhanced adhesion of Ms to murine macrophages and human epithelial cells and improved bacterial persistence in spleen tissue following infection in mice. Expression of the PE_PGRS3 functional deletion mutant lacking the C-terminal domain in Ms did not enhance adhesion to host cells, showing a phenotype similar to the Ms parental strain. Interestingly, enhanced persistence of Ms expressing PE_PGRS3 did not correlate with increased concentrations of inflammatory cytokines. These results point to a critical role for the ≈ 80 amino acids long, arginine-rich C-terminal domain of PE_PGRS3 in tuberculosis pathogenesis." @default.
• W2890427044 created "2018-09-27" @default.
• W2890427044 creator A5005270626 @default.
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• W2890427044 creator A5084050042 @default.
• W2890427044 creator A5084427308 @default.
• W2890427044 date "2018-09-26" @default.
• W2890427044 modified "2023-10-03" @default.
• W2890427044 title "PE_PGRS3 of<i>Mycobacterium tuberculosis</i>is specifically expressed at low phosphate concentration, and its arginine-rich C-terminal domain mediates adhesion and persistence in host tissues when expressed in<i>Mycobacterium smegmatis</i>" @default.
• W2890427044 cites W130282484 @default.
• W2890427044 cites W1551684330 @default.
• W2890427044 cites W1576424885 @default.
• W2890427044 cites W1618386398 @default.
• W2890427044 cites W1735934411 @default.
• W2890427044 cites W1786268878 @default.
• W2890427044 cites W1830032345 @default.
• W2890427044 cites W1839665474 @default.
• W2890427044 cites W1898863040 @default.
• W2890427044 cites W1969555953 @default.
• W2890427044 cites W1969623044 @default.
• W2890427044 cites W1974305305 @default.
• W2890427044 cites W1976656389 @default.
• W2890427044 cites W1982648171 @default.
• W2890427044 cites W1991636630 @default.
• W2890427044 cites W1992027784 @default.
• W2890427044 cites W2007752039 @default.
• W2890427044 cites W2017173637 @default.
• W2890427044 cites W2027399746 @default.
• W2890427044 cites W2033333560 @default.
• W2890427044 cites W2036766369 @default.
• W2890427044 cites W2041166598 @default.
• W2890427044 cites W2044657214 @default.
• W2890427044 cites W2051139401 @default.
• W2890427044 cites W2055224823 @default.
• W2890427044 cites W2063986522 @default.
• W2890427044 cites W2067092006 @default.
• W2890427044 cites W2069375058 @default.
• W2890427044 cites W2069488810 @default.
• W2890427044 cites W2072602445 @default.
• W2890427044 cites W2078290231 @default.
• W2890427044 cites W2088316319 @default.
• W2890427044 cites W2094584704 @default.
• W2890427044 cites W2097186842 @default.
• W2890427044 cites W2099106948 @default.
• W2890427044 cites W2100887397 @default.
• W2890427044 cites W2101533192 @default.
• W2890427044 cites W2102202108 @default.
• W2890427044 cites W2102906662 @default.
• W2890427044 cites W2116738290 @default.
• W2890427044 cites W2122947253 @default.
• W2890427044 cites W2125112523 @default.
• W2890427044 cites W2130004041 @default.
• W2890427044 cites W2135524542 @default.
• W2890427044 cites W2135909833 @default.
• W2890427044 cites W2137614072 @default.
• W2890427044 cites W2139399320 @default.
• W2890427044 cites W2141166469 @default.
• W2890427044 cites W2141920762 @default.
• W2890427044 cites W2145568057 @default.
• W2890427044 cites W2150740864 @default.
• W2890427044 cites W2151874196 @default.
• W2890427044 cites W2152034069 @default.
• W2890427044 cites W2153075964 @default.
• W2890427044 cites W2154293774 @default.
• W2890427044 cites W2156496439 @default.
• W2890427044 cites W2158023121 @default.
• W2890427044 cites W2225338927 @default.
• W2890427044 cites W2279580203 @default.
• W2890427044 cites W2294741826 @default.
• W2890427044 cites W2298051754 @default.
• W2890427044 cites W2338145181 @default.
• W2890427044 cites W2338769870 @default.
• W2890427044 cites W2344623542 @default.
• W2890427044 cites W2344958379 @default.
• W2890427044 cites W2405978957 @default.
• W2890427044 cites W2476497360 @default.
• W2890427044 cites W2509271126 @default.
• W2890427044 cites W2587971454 @default.
• W2890427044 cites W2609244548 @default.
• W2890427044 cites W2609380138 @default.
• W2890427044 cites W2747048288 @default.
• W2890427044 cites W2767640841 @default.
• W2890427044 cites W2784014905 @default.
• W2890427044 cites W4210627381 @default.
• W2890427044 doi "https://doi.org/10.1111/cmi.12952" @default.
• W2890427044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30192424" @default.

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