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- W2890462765 abstract "Plant piercing sucking insects mainly feed on phloem sap containing a high amount of sucrose. To enhance the absorption of sucrose from the midgut, sucrose hydrolase digests sucrose into glucose and fructose. In this study, a sucrose hydrolase homolog (DcSuh) was identified and targeted in Diaphorina citri, the vector of huanglongbing (HLB), by RNA interference (RNAi). In silico analysis revealed the presence of an Aamy domain in the DcSUH protein, which is characteristic of the glycoside hydrolase family 13 (GH13). Phylogenetic analysis showed DcSuh was closely related to the sucrose hydrolase of other Hemiptera members. The highest gene expression levels of DcSuh was found in the 4th and 5th instar nymphs. dsRNA-mediated RNAi of DcSuh was achieved through topical feeding. Our results showed that application of 0.2 μL of 500 ng μL−1 (100 ng) dsRNA-DcSuh was sufficient to repress the expression of the targeted gene and cause nymph mortality and reduce adult lifespan. The reduction in gene expression, mortality, and lifespan was dose-dependent. In agreement with the gene expression results, treatment with dsRNA-DcSuh significantly reduced sucrose hydrolase activity in treated nymphs and emerged adults from treated nymphs. Interestingly, some emerged adults from treated nymphs showed a swollen abdomen phenotype, indicating that these insects were under osmotic stress. Although the percentage of swollen abdomens was low, their incidence was significantly correlated with the concentration of applied dsRNA-DcSuh. Metabolomic analyses using GC-MS showed an accumulation of sucrose and a reduction in fructose, glucose and trehalose in treated nymphs, confirming the inhibition of sucrose hydrolase activity. Additionally, most of the secondary metabolites were reduced in the treated nymphs, indicating a reduction in the biological activities in D. citri and that they are under stress. Our findings indicate that sucrose hydrolase might be a potential target for effective RNAi control of D. citri." @default.
- W2890462765 created "2018-09-27" @default.
- W2890462765 creator A5022756749 @default.
- W2890462765 creator A5063714269 @default.
- W2890462765 date "2018-10-01" @default.
- W2890462765 modified "2023-10-16" @default.
- W2890462765 title "Silencing of sucrose hydrolase causes nymph mortality and disturbs adult osmotic homeostasis in Diaphorina citri (Hemiptera: Liviidae)" @default.
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- W2890462765 doi "https://doi.org/10.1016/j.ibmb.2018.09.003" @default.
- W2890462765 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30205149" @default.
- W2890462765 hasPublicationYear "2018" @default.