Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890530640> ?p ?o ?g. }
- W2890530640 abstract "Abstract Positive-sense RNA viruses hijack intracellular membranes that provide niches for viral RNA synthesis and a platform for interactions with host proteins. However, little is known about host factors at the interface between replicase complexes and the host cytoplasm. We engineered a biotin ligase into a coronaviral replication/transcription complex (RTC) and identified >500 host proteins constituting the RTC microenvironment. siRNA-silencing of each RTC-proximal host factor demonstrated importance of vesicular trafficking pathways, ubiquitin-dependent and autophagy-related processes, and translation initiation factors. Notably, detection of translation initiation factors at the RTC was instrumental to visualize and demonstrate active translation proximal to replication complexes of several coronaviruses. Collectively, we establish a spatial link between viral RNA synthesis and diverse host factors of unprecedented breadth. Our data may serve as a paradigm for other positive-strand RNA viruses and provide a starting point for a comprehensive analysis of critical virus-host interactions that represent targets for therapeutic intervention." @default.
- W2890530640 created "2018-09-27" @default.
- W2890530640 creator A5004252677 @default.
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- W2890530640 creator A5083265125 @default.
- W2890530640 creator A5083568300 @default.
- W2890530640 date "2018-09-14" @default.
- W2890530640 modified "2023-10-01" @default.
- W2890530640 title "Determination of host cell proteins constituting the molecular microenvironment of coronavirus replicase complexes by proximity-labeling" @default.
- W2890530640 cites W1491556421 @default.
- W2890530640 cites W1522319173 @default.
- W2890530640 cites W1594208479 @default.
- W2890530640 cites W1639583514 @default.
- W2890530640 cites W1854727002 @default.
- W2890530640 cites W1970341475 @default.
- W2890530640 cites W1970395554 @default.
- W2890530640 cites W1971214320 @default.
- W2890530640 cites W1972361086 @default.
- W2890530640 cites W1992496728 @default.
- W2890530640 cites W2006229859 @default.
- W2890530640 cites W2008410413 @default.
- W2890530640 cites W2008641356 @default.
- W2890530640 cites W2010692119 @default.
- W2890530640 cites W2022879707 @default.
- W2890530640 cites W2023949937 @default.
- W2890530640 cites W2030550897 @default.
- W2890530640 cites W2036693329 @default.
- W2890530640 cites W2040154453 @default.
- W2890530640 cites W2040208749 @default.
- W2890530640 cites W2040676413 @default.
- W2890530640 cites W2043464646 @default.
- W2890530640 cites W2047078583 @default.
- W2890530640 cites W2051293392 @default.
- W2890530640 cites W2053699655 @default.
- W2890530640 cites W2054230277 @default.
- W2890530640 cites W2064612794 @default.
- W2890530640 cites W2071680235 @default.
- W2890530640 cites W2071939999 @default.
- W2890530640 cites W2072904045 @default.
- W2890530640 cites W2081997160 @default.
- W2890530640 cites W2083162899 @default.
- W2890530640 cites W2087200538 @default.
- W2890530640 cites W2092581586 @default.
- W2890530640 cites W2095928865 @default.
- W2890530640 cites W2096144498 @default.
- W2890530640 cites W2096173332 @default.
- W2890530640 cites W2099866950 @default.
- W2890530640 cites W2101990520 @default.
- W2890530640 cites W2103017472 @default.
- W2890530640 cites W2104765679 @default.
- W2890530640 cites W2105707485 @default.
- W2890530640 cites W2107277218 @default.
- W2890530640 cites W2113457186 @default.
- W2890530640 cites W2122550753 @default.
- W2890530640 cites W2123799993 @default.
- W2890530640 cites W2126428749 @default.
- W2890530640 cites W2131848047 @default.
- W2890530640 cites W2133256064 @default.
- W2890530640 cites W2133465414 @default.
- W2890530640 cites W2134168236 @default.
- W2890530640 cites W2141545183 @default.
- W2890530640 cites W2145845216 @default.
- W2890530640 cites W2149613436 @default.
- W2890530640 cites W2151763439 @default.
- W2890530640 cites W2154551219 @default.
- W2890530640 cites W2154850912 @default.
- W2890530640 cites W2156681639 @default.
- W2890530640 cites W2157658519 @default.
- W2890530640 cites W2158217645 @default.
- W2890530640 cites W2159857626 @default.
- W2890530640 cites W2160620965 @default.
- W2890530640 cites W2161770119 @default.
- W2890530640 cites W2171291163 @default.
- W2890530640 cites W2174898867 @default.
- W2890530640 cites W2238516932 @default.
- W2890530640 cites W2252997710 @default.
- W2890530640 cites W2419079509 @default.
- W2890530640 cites W2426204902 @default.
- W2890530640 cites W2439020788 @default.
- W2890530640 cites W2531764546 @default.
- W2890530640 cites W2537623931 @default.
- W2890530640 cites W2583423445 @default.
- W2890530640 cites W2584597755 @default.
- W2890530640 cites W2591941343 @default.
- W2890530640 cites W2592647402 @default.
- W2890530640 cites W2604808360 @default.
- W2890530640 cites W2612768906 @default.
- W2890530640 cites W2742542317 @default.
- W2890530640 cites W2769072089 @default.