Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890534722> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2890534722 abstract "e15081 Background: Changes in microRNA (miRNA) expression during treatment for locally advanced rectal cancer (LARC) may provide insight into disease biology and potentially act as predictive biomarkers. We investigated 112 miRNAs in serum during neoadjuvant chemoradiation for LARC. Methods: Serum was collected at baseline, week 3 and at completion of chemoradiation from 40 prospectively recruited patients with LARC. Responders were classed as tumour regression grade (AJCC classification) 0 or 1 and non-responders as 2 or 3. Serum was also collected from 20 healthy controls. RNA extraction was performed using the Norgen total RNA purification kit. Reverse transcription and pre-amplification were performed according to Taqman OpenArray MicroRNA Panels manufacturer's instructions. QuantStudio12K platform was used for miRNA array qPCR. The delta-delta-Ct method was used to identifiy differentially expressed miRNAs, normalised against U6 snRNA. Analysis was performed in R using paired t-statistics and the Benjamini-Hochberg False Discovery Rate for multiple hypothesis testing adjustment, with q < 0.05 for significance. Enriched KEGG pathways were identified using DIANA, based on verified gene targets of each miRNA from Tarbase. Results: Four miRNAs (miR-125b-1, miR-1183, miR-130a, miR-375) were differentially expressed in baseline patient samples compared to controls. From baseline to completion of treatment, three of these - miR-125b-1, miR-1183, miR-130a were downregulated by more than 2-fold. Comparing responders and non-responders, miR-130a was significantly downregulated in the non-responders only. Conclusions: miR-125b-1, miR-1183 and miR-130a are significantly downregulated in patients with LARC during chemoradiation. These miRNAs are known to target key colorectal cancer genes such as ATM and CHEK1, which have been implicated in chemoradiation resistance. miR-130a warrants further investigation as a predictive biomarker, being downregulated in patients with a poor response to therapy. Work is ongoing, investigating these miRNA targets in the solid tissue in these patients. To our knowledge, this is the first study to profile potentially predictive miRNA changes during chemoradiation in LARC." @default.
- W2890534722 created "2018-09-27" @default.
- W2890534722 creator A5012845099 @default.
- W2890534722 creator A5015202246 @default.
- W2890534722 creator A5017825565 @default.
- W2890534722 creator A5025720772 @default.
- W2890534722 creator A5034573171 @default.
- W2890534722 creator A5054480788 @default.
- W2890534722 creator A5056450681 @default.
- W2890534722 creator A5064553867 @default.
- W2890534722 creator A5064866788 @default.
- W2890534722 creator A5071226647 @default.
- W2890534722 creator A5083207399 @default.
- W2890534722 date "2017-05-20" @default.
- W2890534722 modified "2023-09-26" @default.
- W2890534722 title "Serum microRNA expression during neoadjuvant chemoradiation for rectal cancer." @default.
- W2890534722 doi "https://doi.org/10.1200/jco.2017.35.15_suppl.e15081" @default.
- W2890534722 hasPublicationYear "2017" @default.
- W2890534722 type Work @default.
- W2890534722 sameAs 2890534722 @default.
- W2890534722 citedByCount "3" @default.
- W2890534722 countsByYear W28905347222019 @default.
- W2890534722 countsByYear W28905347222020 @default.
- W2890534722 countsByYear W28905347222022 @default.
- W2890534722 crossrefType "journal-article" @default.
- W2890534722 hasAuthorship W2890534722A5012845099 @default.
- W2890534722 hasAuthorship W2890534722A5015202246 @default.
- W2890534722 hasAuthorship W2890534722A5017825565 @default.
- W2890534722 hasAuthorship W2890534722A5025720772 @default.
- W2890534722 hasAuthorship W2890534722A5034573171 @default.
- W2890534722 hasAuthorship W2890534722A5054480788 @default.
- W2890534722 hasAuthorship W2890534722A5056450681 @default.
- W2890534722 hasAuthorship W2890534722A5064553867 @default.
- W2890534722 hasAuthorship W2890534722A5064866788 @default.
- W2890534722 hasAuthorship W2890534722A5071226647 @default.
- W2890534722 hasAuthorship W2890534722A5083207399 @default.
- W2890534722 hasConcept C104317684 @default.
- W2890534722 hasConcept C121608353 @default.
- W2890534722 hasConcept C126322002 @default.
- W2890534722 hasConcept C143998085 @default.
- W2890534722 hasConcept C145059251 @default.
- W2890534722 hasConcept C185592680 @default.
- W2890534722 hasConcept C2778292576 @default.
- W2890534722 hasConcept C502942594 @default.
- W2890534722 hasConcept C526805850 @default.
- W2890534722 hasConcept C530470458 @default.
- W2890534722 hasConcept C55493867 @default.
- W2890534722 hasConcept C71924100 @default.
- W2890534722 hasConceptScore W2890534722C104317684 @default.
- W2890534722 hasConceptScore W2890534722C121608353 @default.
- W2890534722 hasConceptScore W2890534722C126322002 @default.
- W2890534722 hasConceptScore W2890534722C143998085 @default.
- W2890534722 hasConceptScore W2890534722C145059251 @default.
- W2890534722 hasConceptScore W2890534722C185592680 @default.
- W2890534722 hasConceptScore W2890534722C2778292576 @default.
- W2890534722 hasConceptScore W2890534722C502942594 @default.
- W2890534722 hasConceptScore W2890534722C526805850 @default.
- W2890534722 hasConceptScore W2890534722C530470458 @default.
- W2890534722 hasConceptScore W2890534722C55493867 @default.
- W2890534722 hasConceptScore W2890534722C71924100 @default.
- W2890534722 hasLocation W28905347221 @default.
- W2890534722 hasOpenAccess W2890534722 @default.
- W2890534722 hasPrimaryLocation W28905347221 @default.
- W2890534722 hasRelatedWork W2012871937 @default.
- W2890534722 hasRelatedWork W2043126238 @default.
- W2890534722 hasRelatedWork W2087844268 @default.
- W2890534722 hasRelatedWork W2242925929 @default.
- W2890534722 hasRelatedWork W2273296906 @default.
- W2890534722 hasRelatedWork W2326922953 @default.
- W2890534722 hasRelatedWork W2758112813 @default.
- W2890534722 hasRelatedWork W2759185715 @default.
- W2890534722 hasRelatedWork W2897685529 @default.
- W2890534722 hasRelatedWork W2970425180 @default.
- W2890534722 hasRelatedWork W2981291970 @default.
- W2890534722 hasRelatedWork W2990630116 @default.
- W2890534722 hasRelatedWork W2999398223 @default.
- W2890534722 hasRelatedWork W3004835645 @default.
- W2890534722 hasRelatedWork W3015009822 @default.
- W2890534722 hasRelatedWork W3022296573 @default.
- W2890534722 hasRelatedWork W3128257440 @default.
- W2890534722 hasRelatedWork W3129030142 @default.
- W2890534722 hasRelatedWork W3202667652 @default.
- W2890534722 hasRelatedWork W3024187595 @default.
- W2890534722 isParatext "false" @default.
- W2890534722 isRetracted "false" @default.
- W2890534722 magId "2890534722" @default.
- W2890534722 workType "article" @default.