FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890558611> ?p ?o ?g. }

• W2890558611 endingPage "10468" @default.
• W2890558611 startingPage "10456" @default.
• W2890558611 abstract "Essential amino acids (EAA) play an important role in promoting milk protein synthesis in primary bovine mammary epithelial cells (BMEC). However, the regulatory mechanisms involved in the relationship between EAA and milk protein synthesis have not been fully explored. This study examined the effects of seryl-tRNA synthetase (SARS) on EAA-stimulated β-casein synthesis, cell proliferation, and the mammalian target of rapamycin (mTOR) system in BMEC. First, BMEC were cultured in medium either lacking all EAA (−EAA) or that included all EAA (+EAA) for 12 h. The BMEC were then supplemented with the opposing treatments (−EAA supplemented with +EAA and vice versa) for 0 h, 10 min, 0.5 h, 1 h, 6 h, or 12 h, respectively. After the treatment-specific time allotment, proteins were collected for Western blotting. Subsequently, a 2 × 2 factorial design was used to evaluate the interactive of SARS inhibition (control or SARS inhibited) and EAA supply (+EAA or –EAA) on gene and protein abundance, cell viability, and cell cycle in BMEC. Based on the data obtained in the first experiment, the changes in protein abundance of β-casein and SARS depended on EAA treatment time in similar patterns. The protein abundance of β-casein, SARS, and mammalian target of rapamycin (mTOR)-related proteins, cell viability, cell cycle progression, and the mRNA abundance of cyclin D1 (CCND1, cell cycle progression marker) and marker of proliferation Ki-67 (MKI67, cell proliferation marker) were stimulated by the presence of EAA. Correspondingly, when cells were deprived of EAA, cell proliferation and abundance of these proteins and genes were reduced overall. Moreover, the decreases in these aspects were further exacerbated by inhibiting SARS, suggesting that an interaction between EAA and SARS is important for regulating protein synthesis. The results indicated that SARS stimulated the mTOR signaling pathway when EAA were present, enhanced EAA-stimulated cell proliferation, and contributed to increased β-casein production in BMEC." @default.
• W2890558611 created "2018-09-27" @default.
• W2890558611 creator A5005375595 @default.
• W2890558611 creator A5007394179 @default.
• W2890558611 creator A5075760641 @default.
• W2890558611 creator A5080239275 @default.
• W2890558611 date "2018-11-01" @default.
• W2890558611 modified "2023-09-29" @default.
• W2890558611 title "Seryl-tRNA synthetase-mediated essential amino acids regulate β-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells" @default.
• W2890558611 cites W1911697332 @default.
• W2890558611 cites W1959856935 @default.
• W2890558611 cites W1967164805 @default.
• W2890558611 cites W1977534471 @default.
• W2890558611 cites W1986665517 @default.
• W2890558611 cites W1992735813 @default.
• W2890558611 cites W2008239768 @default.
• W2890558611 cites W2012130540 @default.
• W2890558611 cites W2018651892 @default.
• W2890558611 cites W2022984399 @default.
• W2890558611 cites W2023344759 @default.
• W2890558611 cites W2040223620 @default.
• W2890558611 cites W2041031771 @default.
• W2890558611 cites W2041228471 @default.
• W2890558611 cites W2045812968 @default.
• W2890558611 cites W2051648135 @default.
• W2890558611 cites W2051652691 @default.
• W2890558611 cites W2056664023 @default.
• W2890558611 cites W2074996957 @default.
• W2890558611 cites W2077587813 @default.
• W2890558611 cites W2078602113 @default.
• W2890558611 cites W2103590517 @default.
• W2890558611 cites W2107277218 @default.
• W2890558611 cites W2108797553 @default.
• W2890558611 cites W2112189902 @default.
• W2890558611 cites W2119418779 @default.
• W2890558611 cites W2120861858 @default.
• W2890558611 cites W2126334171 @default.
• W2890558611 cites W2129260297 @default.
• W2890558611 cites W2132531320 @default.
• W2890558611 cites W2134862369 @default.
• W2890558611 cites W2144687234 @default.
• W2890558611 cites W2315610230 @default.
• W2890558611 cites W2398251883 @default.
• W2890558611 cites W2607481827 @default.
• W2890558611 cites W2608910584 @default.
• W2890558611 cites W2619187960 @default.
• W2890558611 cites W2708158780 @default.
• W2890558611 cites W2781385814 @default.
• W2890558611 cites W2807262847 @default.
• W2890558611 cites W914670989 @default.
• W2890558611 doi "https://doi.org/10.3168/jds.2018-14568" @default.
• W2890558611 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30219419" @default.
• W2890558611 hasPublicationYear "2018" @default.
• W2890558611 type Work @default.
• W2890558611 sameAs 2890558611 @default.
• W2890558611 citedByCount "11" @default.
• W2890558611 countsByYear W28905586112019 @default.
• W2890558611 countsByYear W28905586112020 @default.
• W2890558611 countsByYear W28905586112021 @default.
• W2890558611 countsByYear W28905586112022 @default.
• W2890558611 countsByYear W28905586112023 @default.
• W2890558611 crossrefType "journal-article" @default.
• W2890558611 hasAuthorship W2890558611A5005375595 @default.
• W2890558611 hasAuthorship W2890558611A5007394179 @default.
• W2890558611 hasAuthorship W2890558611A5075760641 @default.
• W2890558611 hasAuthorship W2890558611A5080239275 @default.
• W2890558611 hasBestOaLocation W28905586111 @default.
• W2890558611 hasConcept C1491633281 @default.
• W2890558611 hasConcept C153911025 @default.
• W2890558611 hasConcept C199835354 @default.
• W2890558611 hasConcept C29537977 @default.
• W2890558611 hasConcept C53227056 @default.
• W2890558611 hasConcept C55493867 @default.
• W2890558611 hasConcept C62112901 @default.
• W2890558611 hasConcept C62478195 @default.
• W2890558611 hasConcept C86554907 @default.
• W2890558611 hasConcept C86803240 @default.
• W2890558611 hasConcept C95444343 @default.
• W2890558611 hasConceptScore W2890558611C1491633281 @default.
• W2890558611 hasConceptScore W2890558611C153911025 @default.
• W2890558611 hasConceptScore W2890558611C199835354 @default.
• W2890558611 hasConceptScore W2890558611C29537977 @default.
• W2890558611 hasConceptScore W2890558611C53227056 @default.
• W2890558611 hasConceptScore W2890558611C55493867 @default.
• W2890558611 hasConceptScore W2890558611C62112901 @default.
• W2890558611 hasConceptScore W2890558611C62478195 @default.
• W2890558611 hasConceptScore W2890558611C86554907 @default.
• W2890558611 hasConceptScore W2890558611C86803240 @default.
• W2890558611 hasConceptScore W2890558611C95444343 @default.
• W2890558611 hasIssue "11" @default.
• W2890558611 hasLocation W28905586111 @default.
• W2890558611 hasLocation W28905586112 @default.
• W2890558611 hasOpenAccess W2890558611 @default.
• W2890558611 hasPrimaryLocation W28905586111 @default.
• W2890558611 hasRelatedWork W2013115645 @default.
• W2890558611 hasRelatedWork W2083693850 @default.
• W2890558611 hasRelatedWork W2348042013 @default.
• W2890558611 hasRelatedWork W2382814410 @default.

• next