FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890569696> ?p ?o ?g. }

• W2890569696 endingPage "37" @default.
• W2890569696 startingPage "25" @default.
• W2890569696 abstract "Natural killer (NK) cells are essential players of the innate immune response that secrete cytolytic factors and cytokines such as IFN-γ when contacting virus-infected or tumor cells. They represent prime targets in immunotherapy as defects in NK cell functions are hallmarks of many pathological conditions, such as cancer and chronic infections. The functional screening of chemical libraries or biologics would greatly help identify new modulators of NK cell activity, but commonly used methods such as flow cytometry are not easily scalable to high-throughput settings. Here we describe an efficient assay to measure the natural cytotoxicity of primary NK cells where the bioluminescent enzyme NanoLuc is constitutively expressed in the cytoplasm of target cells and is released in co-culture supernatants when lysis occurs. We fully characterized this assay using either purified NK cells or total peripheral blood mononuclear cells (PBMCs), including some patient samples, as effector cells. A pilot screen was also performed on a library of 782 metabolites, xenobiotics, and common drugs, which identified dextrometorphan and diphenhydramine as novel NK cell inhibitors. Finally, this assay was further improved by developing a dual-reporter cell line to simultaneously measure NK cell cytotoxicity and IFN-γ secretion in a single well, extending the potential of this system." @default.
• W2890569696 created "2018-09-27" @default.
• W2890569696 creator A5003573061 @default.
• W2890569696 creator A5008851525 @default.
• W2890569696 creator A5015831996 @default.
• W2890569696 creator A5017865997 @default.
• W2890569696 creator A5019383417 @default.
• W2890569696 creator A5023269916 @default.
• W2890569696 creator A5027527369 @default.
• W2890569696 creator A5030796777 @default.
• W2890569696 creator A5050620583 @default.
• W2890569696 creator A5060157202 @default.
• W2890569696 creator A5061140176 @default.
• W2890569696 creator A5073503843 @default.
• W2890569696 creator A5079484759 @default.
• W2890569696 creator A5080355953 @default.
• W2890569696 date "2019-01-01" @default.
• W2890569696 modified "2023-10-16" @default.
• W2890569696 title "Identification of Primary Natural Killer Cell Modulators by Chemical Library Screening with a Luciferase-Based Functional Assay" @default.
• W2890569696 cites W1516464304 @default.
• W2890569696 cites W1937833096 @default.
• W2890569696 cites W1964963939 @default.
• W2890569696 cites W1967939624 @default.
• W2890569696 cites W1970877840 @default.
• W2890569696 cites W1973156541 @default.
• W2890569696 cites W1975118460 @default.
• W2890569696 cites W1987983325 @default.
• W2890569696 cites W1988382766 @default.
• W2890569696 cites W1991001144 @default.
• W2890569696 cites W1995284402 @default.
• W2890569696 cites W1998985248 @default.
• W2890569696 cites W2014105188 @default.
• W2890569696 cites W2022069273 @default.
• W2890569696 cites W2025041034 @default.
• W2890569696 cites W2027200716 @default.
• W2890569696 cites W2027608934 @default.
• W2890569696 cites W2039751255 @default.
• W2890569696 cites W2057682244 @default.
• W2890569696 cites W2071587244 @default.
• W2890569696 cites W2072207738 @default.
• W2890569696 cites W2073300038 @default.
• W2890569696 cites W2075464097 @default.
• W2890569696 cites W2077699378 @default.
• W2890569696 cites W2085091421 @default.
• W2890569696 cites W2090207272 @default.
• W2890569696 cites W2095827366 @default.
• W2890569696 cites W2104825199 @default.
• W2890569696 cites W2108768020 @default.
• W2890569696 cites W2117222092 @default.
• W2890569696 cites W2134453830 @default.
• W2890569696 cites W2135144563 @default.
• W2890569696 cites W2257443024 @default.
• W2890569696 cites W2287383446 @default.
• W2890569696 cites W2406043212 @default.
• W2890569696 cites W2586736965 @default.
• W2890569696 cites W2753189779 @default.
• W2890569696 cites W2781789112 @default.
• W2890569696 doi "https://doi.org/10.1177/2472555218797078" @default.
• W2890569696 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30184441" @default.
• W2890569696 hasPublicationYear "2019" @default.
• W2890569696 type Work @default.
• W2890569696 sameAs 2890569696 @default.
• W2890569696 citedByCount "10" @default.
• W2890569696 countsByYear W28905696962020 @default.
• W2890569696 countsByYear W28905696962021 @default.
• W2890569696 countsByYear W28905696962022 @default.
• W2890569696 countsByYear W28905696962023 @default.
• W2890569696 crossrefType "journal-article" @default.
• W2890569696 hasAuthorship W2890569696A5003573061 @default.
• W2890569696 hasAuthorship W2890569696A5008851525 @default.
• W2890569696 hasAuthorship W2890569696A5015831996 @default.
• W2890569696 hasAuthorship W2890569696A5017865997 @default.
• W2890569696 hasAuthorship W2890569696A5019383417 @default.
• W2890569696 hasAuthorship W2890569696A5023269916 @default.
• W2890569696 hasAuthorship W2890569696A5027527369 @default.
• W2890569696 hasAuthorship W2890569696A5030796777 @default.
• W2890569696 hasAuthorship W2890569696A5050620583 @default.
• W2890569696 hasAuthorship W2890569696A5060157202 @default.
• W2890569696 hasAuthorship W2890569696A5061140176 @default.
• W2890569696 hasAuthorship W2890569696A5073503843 @default.
• W2890569696 hasAuthorship W2890569696A5079484759 @default.
• W2890569696 hasAuthorship W2890569696A5080355953 @default.
• W2890569696 hasBestOaLocation W28905696961 @default.
• W2890569696 hasConcept C109316439 @default.
• W2890569696 hasConcept C129374314 @default.
• W2890569696 hasConcept C137061746 @default.
• W2890569696 hasConcept C1491633281 @default.
• W2890569696 hasConcept C202751555 @default.
• W2890569696 hasConcept C203014093 @default.
• W2890569696 hasConcept C2776090121 @default.
• W2890569696 hasConcept C2778102761 @default.
• W2890569696 hasConcept C28651165 @default.
• W2890569696 hasConcept C54355233 @default.
• W2890569696 hasConcept C553184892 @default.
• W2890569696 hasConcept C55493867 @default.
• W2890569696 hasConcept C81885089 @default.
• W2890569696 hasConcept C86803240 @default.
• W2890569696 hasConcept C8891405 @default.

• next