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• W2890576302 abstract "Oxidation of hydroxamic acids (HXs) generates HNO, and it is not clear whether it is formed also in the presence of metal ions. The kinetics of the oxidation of HXs, such as acetohydroxamic acid, suberohydroxamic acid, and suberoylanilide hydroxamic acid (SAHA), by compounds I and II of horseradish peroxidase (HRP) at pH 7.0 and 25 °C have been studied using rapid-mixing stopped-flow. The kinetics of these reactions were compared to those observed in the presence of Cu(ClO4)2, NiSO4, or ZnSO4. The rates decrease upon increasing [CuII] at constant [HXs], and no oxidation of HX occurs when [HX]/[CuII] ≈ 2, implying that HX oxidation in the presence of CuII proceeds through the free ligand since the predominant complex is CuX2. In the case of NiII, the oxidation rate decreases upon increasing the ratio [NiII]/[HX] beyond 1, where the predominant complex is NiIIX+, implying that its oxidation is feasible. The effect of ZnII could be studied only on the rate of HXs oxidation by compound II demonstrating similar behavior to that of NiII. HXs were also oxidized catalytically by HRP/H2O2 at pH 7.0, demonstrating that metal ions facilitate the formation of HNO while hardly affecting its yield and the extent of HX oxidation." @default.
• W2890576302 created "2018-09-27" @default.
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• W2890576302 date "2018-07-03" @default.
• W2890576302 modified "2023-09-24" @default.
• W2890576302 title "A kinetic study of the oxidation of hydroxamic acids by compounds I and II of horseradish peroxidase: Effect of transition metal ions" @default.
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• W2890576302 doi "https://doi.org/10.1080/00958972.2018.1493200" @default.
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