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• W2890588319 abstract "Morbidity and mortality attributed to Clostridium difficile infection (CDI) have increased over the past 20 years. Currently, antibiotics are the only US FDA-approved treatment for primary C. difficile infection, and these are, ironically, associated with disease relapse and the threat of burgeoning drug resistance. We previously showed that non-toxin virulence factors play key roles in CDI, and that colonization factors are critical for disease. Specifically, a C. difficile adhesin, Surface Layer Protein A (SlpA) is a major contributor to host cell attachment. In this work, we engineered Syn-LAB 2.0 and Syn-LAB 2.1, two synthetic biologic agents derived from lactic acid bacteria, to stably and constitutively express a host-cell binding fragment of the C. difficile adhesin SlpA on their cell-surface. Both agents harbor conditional suicide plasmids expressing a codon-optimized chimera of the lactic acid bacterium's cell-wall anchoring surface-protein domain, fused to the conserved, highly adherent, host-cell-binding domain of C. difficile SlpA. Both agents also incorporate engineered biocontrol, obviating the need for any antibiotic selection. Syn-LAB 2.0 and Syn-LAB 2.1 possess positive biophysical and in vivo properties compared with their parental antecedents in that they robustly and constitutively display the SlpA chimera on their cell surface, potentiate human intestinal epithelial barrier function in vitro, are safe, tolerable and palatable to Golden Syrian hamsters and neonatal piglets at high daily doses, and are detectable in animal feces within 24 h of dosing, confirming robust colonization. In combination, the engineered strains also delay (in fixed doses) or prevent (when continuously administered) death of infected hamsters upon challenge with high doses of virulent C. difficile. Finally, fixed-dose Syn-LAB ameliorates diarrhea in a non-lethal model of neonatal piglet enteritis. Taken together, our findings suggest that the two synthetic biologics may be effectively employed as non-antibiotic interventions for CDI." @default.
• W2890588319 created "2018-09-27" @default.
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• W2890588319 date "2018-09-05" @default.
• W2890588319 modified "2023-10-15" @default.
• W2890588319 title "An Engineered Synthetic Biologic Protects Against Clostridium difficile Infection" @default.
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• W2890588319 cites W1830796093 @default.
• W2890588319 cites W1832887102 @default.
• W2890588319 cites W1967283617 @default.
• W2890588319 cites W1979547207 @default.
• W2890588319 cites W1979954621 @default.
• W2890588319 cites W1983980606 @default.
• W2890588319 cites W1990923074 @default.
• W2890588319 cites W1998272935 @default.
• W2890588319 cites W2004942670 @default.
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• W2890588319 cites W2025024815 @default.
• W2890588319 cites W2026158782 @default.
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• W2890588319 cites W2095017924 @default.
• W2890588319 cites W2097936419 @default.
• W2890588319 cites W2100618300 @default.
• W2890588319 cites W2104518766 @default.
• W2890588319 cites W2105413055 @default.
• W2890588319 cites W2119114528 @default.
• W2890588319 cites W2124096689 @default.
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• W2890588319 cites W2137035993 @default.
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• W2890588319 cites W2143782107 @default.
• W2890588319 cites W2145526742 @default.
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• W2890588319 cites W2156524114 @default.
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• W2890588319 cites W2235498129 @default.
• W2890588319 cites W2326747618 @default.
• W2890588319 cites W2339497741 @default.
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• W2890588319 cites W2512704070 @default.
• W2890588319 cites W2513533432 @default.
• W2890588319 cites W2570602940 @default.
• W2890588319 cites W2592003794 @default.
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• W2890588319 doi "https://doi.org/10.3389/fmicb.2018.02080" @default.
• W2890588319 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6134020" @default.
• W2890588319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30233548" @default.
• W2890588319 hasPublicationYear "2018" @default.
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