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• W2890591455 abstract "We demonstrate the enhancement of fluorescence emission from a dye, 5-carboxyfluorescein (FAM), which couples with surface plasmons at the spectral channels of excitation and emission. Experiments and calculations revealed that bimetallic (gold-silver) plasmon, as compared to the monometallic ones, allowed such coupling to be enhanced, at both the spectral channels. We achieved the maximum fluorescence enhancement level of 46.5-fold, with markedly high reproducibility (coefficient of variation ~ 0.5%) at a FAM concentration of 10 nM. We also found that higher fluorescence enhancement was more likely to be reproducible. This encourages the use of this technology for practical applications in fluorescence-based biochemical assays. Moreover, we investigated a FAM concentration-dependent enhancement of fluorescence. It was found that fluorescence enhancement decreased and saturated at above 10 nM concentration possibly due to partial photo-bleaching of FAM molecules." @default.
• W2890591455 created "2018-09-27" @default.
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• W2890591455 date "2018-09-12" @default.
• W2890591455 modified "2023-10-10" @default.
• W2890591455 title "Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM)" @default.
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• W2890591455 doi "https://doi.org/10.3390/mi9090460" @default.
• W2890591455 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6187710" @default.
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