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- W2890596726 abstract "Abstract A rapid, cost-effective and easy method that allows on-site determination of the concentration of live and dead bacterial cells using a fibre-based spectroscopic device (the optrode system) is proposed and demonstrated. Identification of live and dead bacteria was achieved by using the commercially available dyes SYTO 9 and propidium iodide, and fluorescence spectra were measured by the optrode. Three spectral processing methods were evaluated for their effectiveness in predicting the original bacterial concentration in the samples: principal components regression (PCR), partial least squares regression (PLSR) and support vector regression (SVR). Without any sample pre-concentration, PCR achieved the most reliable results. It was able to quantify live bacteria from 10 8 down to 10 6.2 bacteria/mL and showed the potential to detect as low as 10 5.7 bacteria/mL. Meanwhile, enumeration of dead bacteria using PCR was achieved between 10 8 and 10 7 bacteria/mL. The general procedures described in this article can be applied or modified for the enumeration of bacteria within populations stained with fluorescent dyes. The optrode is a promising device for the enumeration of live and dead bacterial populations particularly where rapid, on-site measurement and analysis is required." @default.
- W2890596726 created "2018-09-27" @default.
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- W2890596726 date "2019-03-18" @default.
- W2890596726 modified "2023-10-16" @default.
- W2890596726 title "Near real-time enumeration of live and dead bacteria using a fibre-based spectroscopic device" @default.
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- W2890596726 doi "https://doi.org/10.1038/s41598-019-41221-1" @default.
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