Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890642014> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2890642014 abstract "AIDS epidemic has spread to all parts of Indonesia and currently more than 150 countries reported the existence of HIV/AIDS from around the world. Additionally, HIV/AIDS treatment using ARV drugs also find obstacles that must be faced in terms of host, environment and the agent. The objective of this study was to generate lymphocytes TCD4+ that are resistant to HIV infection generate from PBMCs through by deletion of 32 bp CCR5 encoding gene. In principle, this study was done in three steps. First, isolation, culture and purification of lymphocyte TCD4+ from PBMC (Mather, 2008; Rantam, et.al., 2009). Second, lymphocyte TCD4+ characterization by PCR with primer F 5’CAAGTCGAGCGCCCCGCAAGGGG-3, R 5’GTCCGAGTGTGGCTGATCATCC-3 (Thomsen, et.al., 2002; Yuwono, 2006; Hall and Ziedonis 2007; Purwati, et.al., 2009). Third, designing of lymphocyte TCD4+ prototype which was resistant to HIV infection by deletion of 32 bp CCR5 full gene. Results: Twenty-four hours after culture, there were abundant cell growths. TCD4+ lymphocytes from isolated and cultured 10 ml PBMC were found to be 2 x 107. Phenotype characterization of TCD4+ lymphocyte provided positive results, while the genotype showed similarities to that in corresponding gene bank of CCR5 variant A and variant B. Prototype of HIV resistant TCD4+ lymphocytes was made by nucleotide deletions in conserved areas, at position 554-576 bp, using restriction enzymes EcoRI checked using PCR and sequencing. In conclusion, prototype design of HIV resitent TCD4+ lymphocytes is obtained through the deletion of 32 bp CCR5 encoding full gene at GTCAGTATCAATTCTGGAA GAATTT CCAGACA using EcoRI enzyme.Keywords: HIV/AIDS, resistant TCD4+ lymphocytes, mutant 32 bp CCR5, PBMCs, deletion" @default.
- W2890642014 created "2018-09-27" @default.
- W2890642014 creator A5023381694 @default.
- W2890642014 date "2018-05-17" @default.
- W2890642014 modified "2023-09-25" @default.
- W2890642014 title "PROTOTYPE DESIGN OF LYMPHOCYTE TCD4+ RESISTANT AGAINST HIV INFECTION GENERATED FROM PERIPHERAL BLOOD HAEMATOPOIETIC STEM CELL (PBMCs) By DELETION OF 32 bp CCR5 ENCODING GENE" @default.
- W2890642014 cites W1538969072 @default.
- W2890642014 cites W1972984824 @default.
- W2890642014 cites W1981124765 @default.
- W2890642014 cites W1992396018 @default.
- W2890642014 cites W2013638688 @default.
- W2890642014 cites W2026444780 @default.
- W2890642014 cites W2041551971 @default.
- W2890642014 cites W2066547625 @default.
- W2890642014 cites W2090630729 @default.
- W2890642014 cites W2124058652 @default.
- W2890642014 cites W2129437793 @default.
- W2890642014 cites W2583717204 @default.
- W2890642014 doi "https://doi.org/10.20473/jscrte.v1i1.7566" @default.
- W2890642014 hasPublicationYear "2018" @default.
- W2890642014 type Work @default.
- W2890642014 sameAs 2890642014 @default.
- W2890642014 citedByCount "0" @default.
- W2890642014 crossrefType "journal-article" @default.
- W2890642014 hasAuthorship W2890642014A5023381694 @default.
- W2890642014 hasBestOaLocation W28906420141 @default.
- W2890642014 hasConcept C104317684 @default.
- W2890642014 hasConcept C109159458 @default.
- W2890642014 hasConcept C135763542 @default.
- W2890642014 hasConcept C137061746 @default.
- W2890642014 hasConcept C153911025 @default.
- W2890642014 hasConcept C159047783 @default.
- W2890642014 hasConcept C202751555 @default.
- W2890642014 hasConcept C203014093 @default.
- W2890642014 hasConcept C2777761686 @default.
- W2890642014 hasConcept C28328180 @default.
- W2890642014 hasConcept C54355233 @default.
- W2890642014 hasConcept C86803240 @default.
- W2890642014 hasConceptScore W2890642014C104317684 @default.
- W2890642014 hasConceptScore W2890642014C109159458 @default.
- W2890642014 hasConceptScore W2890642014C135763542 @default.
- W2890642014 hasConceptScore W2890642014C137061746 @default.
- W2890642014 hasConceptScore W2890642014C153911025 @default.
- W2890642014 hasConceptScore W2890642014C159047783 @default.
- W2890642014 hasConceptScore W2890642014C202751555 @default.
- W2890642014 hasConceptScore W2890642014C203014093 @default.
- W2890642014 hasConceptScore W2890642014C2777761686 @default.
- W2890642014 hasConceptScore W2890642014C28328180 @default.
- W2890642014 hasConceptScore W2890642014C54355233 @default.
- W2890642014 hasConceptScore W2890642014C86803240 @default.
- W2890642014 hasLocation W28906420141 @default.
- W2890642014 hasOpenAccess W2890642014 @default.
- W2890642014 hasPrimaryLocation W28906420141 @default.
- W2890642014 hasRelatedWork W1541968098 @default.
- W2890642014 hasRelatedWork W1773202802 @default.
- W2890642014 hasRelatedWork W1981464141 @default.
- W2890642014 hasRelatedWork W2002918805 @default.
- W2890642014 hasRelatedWork W2006527288 @default.
- W2890642014 hasRelatedWork W2030970788 @default.
- W2890642014 hasRelatedWork W2055539204 @default.
- W2890642014 hasRelatedWork W2056114344 @default.
- W2890642014 hasRelatedWork W2057175548 @default.
- W2890642014 hasRelatedWork W2121560692 @default.
- W2890642014 hasRelatedWork W2139866054 @default.
- W2890642014 hasRelatedWork W2142109549 @default.
- W2890642014 hasRelatedWork W2166841429 @default.
- W2890642014 hasRelatedWork W2275979138 @default.
- W2890642014 hasRelatedWork W2308454504 @default.
- W2890642014 hasRelatedWork W2363561795 @default.
- W2890642014 hasRelatedWork W3023952797 @default.
- W2890642014 hasRelatedWork W3031717077 @default.
- W2890642014 hasRelatedWork W3045488977 @default.
- W2890642014 hasRelatedWork W3093712542 @default.
- W2890642014 isParatext "false" @default.
- W2890642014 isRetracted "false" @default.
- W2890642014 magId "2890642014" @default.
- W2890642014 workType "article" @default.