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- W2890684893 abstract "Current HPLC methods for analyzing cAMP Phosphodiesterase activity (PDE) use salts, limiting the life of the columns. For this reason, we have developed an improved ion pairing agent free method, using a simple 150 mm C18-hydro column at 30 °C and two phases: (a) water with 0.1% acetic acid and (b) 85/15 w/w MeOH/tetrahydrofuran with 0.1% acetic acid. Using this method the peaks for cAMP and AMP were obtained with good resolution (R ≈ 1.35) and sensitivity (5·10-9 mols) in only 15 min. Moreover, the method was applied to the GMP/cGMP pair obtaining the same sensitivity and resolution (R ≈ 1.38). The precision and accuracy were tested and the method was verified with a Type IV Phosphodiesterase reaction, which produced AMP from cAMP. The method is cleaner and less aggressive, and represents an interesting alternative to currently used methods." @default.
- W2890684893 created "2018-09-27" @default.
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- W2890684893 date "2019-01-01" @default.
- W2890684893 modified "2023-09-24" @default.
- W2890684893 title "An improved “ion pairing agent free” HPLC-RP method for testing cAMP Phosphodiesterase activity" @default.
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- W2890684893 doi "https://doi.org/10.1016/j.talanta.2018.09.058" @default.
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