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- W2890699160 abstract "Interest in the human microbiome has increased dramatically in the last decade. However, much of this research has focused on bacteria, while the composition and roles of their fungal counterparts remain less understood. Furthermore, a variety of methodological approaches have been applied, and the comparability between studies is unclear. This study compared four primer pairs targeting the small subunit (SSU) rRNA (18S), ITS1, ITS2, and large subunit (LSU) rRNA (26S) genomic regions for their ability to accurately characterize fungal communities typical of the human mycobiota. All four target regions of 21 individual fungal mock community taxa were capable of being amplified adequately and sequenced. Mixed mock community analyses revealed marked variability in the ability of each primer pair to accurately characterize a complex community. ITS target regions outperformed LSU and SSU. Of the ITS regions, ITS1 failed to generate sequences for Yarrowia lipolytica and all three Malassezia species when in a mixed community. These findings were further supported in studies of human sinonasal and mouse fecal samples. Based on these analyses, previous studies using ITS1, SSU, or LSU markers may omit key taxa that are identified by the ITS2 marker. Of methods commonly used in human mycobiota studies to date, we recommend selection of the ITS2 marker. Further investigation of more recently developed fungal primer options will be essential to ultimately determine the optimal methodological approach by which future human mycobiota studies ought to be standardized." @default.
- W2890699160 created "2018-09-27" @default.
- W2890699160 creator A5005419095 @default.
- W2890699160 creator A5007833140 @default.
- W2890699160 creator A5009444223 @default.
- W2890699160 creator A5014704218 @default.
- W2890699160 creator A5044826485 @default.
- W2890699160 creator A5068185046 @default.
- W2890699160 creator A5076857866 @default.
- W2890699160 creator A5087530975 @default.
- W2890699160 date "2018-09-19" @default.
- W2890699160 modified "2023-09-30" @default.
- W2890699160 title "Characterizing the Human Mycobiota: A Comparison of Small Subunit rRNA, ITS1, ITS2, and Large Subunit rRNA Genomic Targets" @default.
- W2890699160 cites W1485637191 @default.
- W2890699160 cites W1535945827 @default.
- W2890699160 cites W1543191406 @default.
- W2890699160 cites W1583191282 @default.
- W2890699160 cites W1701869895 @default.
- W2890699160 cites W1978336193 @default.
- W2890699160 cites W1981500982 @default.
- W2890699160 cites W1995674722 @default.
- W2890699160 cites W2003258181 @default.
- W2890699160 cites W2012490955 @default.
- W2890699160 cites W2013956321 @default.
- W2890699160 cites W2019528078 @default.
- W2890699160 cites W2020950421 @default.
- W2890699160 cites W2029127180 @default.
- W2890699160 cites W2029374202 @default.
- W2890699160 cites W2029712882 @default.
- W2890699160 cites W2030311878 @default.
- W2890699160 cites W2032066134 @default.
- W2890699160 cites W2033695145 @default.
- W2890699160 cites W2034285706 @default.
- W2890699160 cites W2041174584 @default.
- W2890699160 cites W2051201019 @default.
- W2890699160 cites W2051572323 @default.
- W2890699160 cites W2053392653 @default.
- W2890699160 cites W2064146024 @default.
- W2890699160 cites W2067003464 @default.
- W2890699160 cites W2069773272 @default.
- W2890699160 cites W2074335138 @default.
- W2890699160 cites W2077088073 @default.
- W2890699160 cites W2082103066 @default.
- W2890699160 cites W2089805675 @default.
- W2890699160 cites W2095667786 @default.
- W2890699160 cites W2097769208 @default.
- W2890699160 cites W2104608591 @default.
- W2890699160 cites W2107331615 @default.
- W2890699160 cites W2107794472 @default.
- W2890699160 cites W2108045667 @default.
- W2890699160 cites W2110375946 @default.
- W2890699160 cites W2111898748 @default.
- W2890699160 cites W2114074735 @default.
- W2890699160 cites W2118155145 @default.
- W2890699160 cites W2118629634 @default.
- W2890699160 cites W2118734291 @default.
- W2890699160 cites W2121636058 @default.
- W2890699160 cites W2121774335 @default.
- W2890699160 cites W2122503382 @default.
- W2890699160 cites W2123024511 @default.
- W2890699160 cites W2123148741 @default.
- W2890699160 cites W2124351063 @default.
- W2890699160 cites W2130889389 @default.
- W2890699160 cites W2132002261 @default.
- W2890699160 cites W2132113202 @default.
- W2890699160 cites W2133053842 @default.
- W2890699160 cites W2134564677 @default.
- W2890699160 cites W2136305645 @default.
- W2890699160 cites W2147637673 @default.
- W2890699160 cites W2151350595 @default.
- W2890699160 cites W2157380302 @default.
- W2890699160 cites W2158379634 @default.
- W2890699160 cites W2162667876 @default.
- W2890699160 cites W2172064173 @default.
- W2890699160 cites W2191014442 @default.
- W2890699160 cites W2345413576 @default.
- W2890699160 cites W2469961355 @default.
- W2890699160 cites W2478983850 @default.
- W2890699160 cites W2518795824 @default.
- W2890699160 cites W2522491625 @default.
- W2890699160 cites W2530689043 @default.
- W2890699160 cites W2538509698 @default.
- W2890699160 cites W2544423525 @default.
- W2890699160 cites W2554506152 @default.
- W2890699160 cites W2557580049 @default.
- W2890699160 cites W2558524460 @default.
- W2890699160 cites W2574474612 @default.
- W2890699160 cites W2587584091 @default.
- W2890699160 cites W2587869072 @default.
- W2890699160 cites W2592537218 @default.
- W2890699160 cites W2611685848 @default.
- W2890699160 cites W2612824952 @default.
- W2890699160 cites W2624678362 @default.
- W2890699160 cites W2731624152 @default.
- W2890699160 cites W2739154049 @default.
- W2890699160 cites W2746672606 @default.
- W2890699160 cites W2746732431 @default.
- W2890699160 cites W2762260969 @default.
- W2890699160 cites W2769232178 @default.
- W2890699160 cites W2769906121 @default.