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• W2890713711 abstract "To show any difference in outcome of laser assisted hatching using 2 different opening sizes on de-vitrified blastocysts. Endpoints were: implantation rate per embryo (IR), clinical pregnancy rate (CPR) and live birth rate (LBR) or on going pregnancy or live birth rate per transfer (LB/OG). Retrospective Study. Embryos were cultured in 30 ul drops of Cook culture media in nunc 60 mm dishes in MINC incubators with 7.0% CO2/5.0% 02 gas. Blastocysts were vitrified on day 5 and/or 6 using I.C.E. vitrification solutions and HSV straws. I.C.E is non DMSO based Ethylene Glycol / Glycerol system. Statistical analysis was performed using Chi-square. Significance was set at P < 0.05. In natural cycle FET, progesterone was initiated the day after LH surge (vaginal endometrin BID or IM p4). Medicated cycles consisting of Estrace + IM or vaginal p4. Embryos were replaced on day 7 of p4. Post thaw embryos were cultured in 30ul drops of BM with 15% HSA < 2 hrs prior to FET. Embryos were hatched with a Hamilton Thorne Zilos 1460 nm IR laser. From 6/1/12 to 9/30/15 a small laser opening (SLO) 10um size, was made with 2-3 pulses of 400 usec duration of 270 uW. From 10/1/15 - 4/6/18 a large laser opening (LLO) 50um size with 15 pulses of 400 usec duration of 270 uW. Embryos were washed 2X in media post hatching. 30 minutes prior to transfer, embryos were put into Embryo Glue. All FET's were ultra sound guided with the Cook Guardia Access embryo transfer catheter. The overall survival rate post de-vitrification was 96.5%. The survival rate for SLO embryos was 96%. SLO was performed on 793 embryos for 535 embryo transfers (FET). The implantation rate per embryo (IR) was 36.9% resulting in a clinical pregnancy rate (CPR) of 42.6% and a live birth rate (LBR) of 33.5%. The survival rate for LLO embryos was 97%. LLO was performed on 902 embryos for 652 FET. The IR was 35.7% per embryo resulting in a clinical pregnancy rate (CPR) of 43.4% and a live birth or on going pregnancy rate (LB/OG) of 34%. Implantation rate (IR) = the number of gestational sacs via ultrasound / the number of embryos transferred. CPR = number of pregnancies documented by ultrasound / the number of FET's. LBR = number of live births / the number of FET's. LB/OG = number of live births + number of ongoing (> 2 us with gestational sac and fetal heart activity + interval growth) / by the number of FET's. From our data, laser hatching over 1,600 de-vitrified blastocysts using I.C.E, there is no statistical difference (p - < 0.05) in implantation rate per embryo, clinical pregnancy per FET, or ongoing and live birth rates per FET with differing sized (10um vs 50 um) zona pellucida opening size.Tabled 1Comparison of Small (SLO) and Large (LLO) Laser openings in 1187 Frozen Embryo TransfersOpening Size# of Embryos Hatched# of FET'sImplantation Rate/Embryo (IR)ClinicalPregnancy Rate (CPR)On Going or DeliveredSLO79353536.9%42.633.5%LLO90265235.7&43.4%35.7%p-value< 0.05 NS< 0.05 NS< 0.05 NS< 0.05 NS< 0.05 NS Open table in a new tab" @default.
• W2890713711 created "2018-09-27" @default.
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• W2890713711 date "2018-09-01" @default.
• W2890713711 modified "2023-09-26" @default.
• W2890713711 title "Does size matter? Comparison of opening size with laser assisted hatching for devitrified blastocysts" @default.
• W2890713711 doi "https://doi.org/10.1016/j.fertnstert.2018.07.670" @default.
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