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• W2890720538 abstract "Abstract Motivation Scaffolding is an essential step during the de novo sequence assembly process to infer the direction and order relationships between the contigs and make the sequence assembly results more continuous and complete. However, scaffolding still faces the challenges of repetitive regions in genome, sequencing errors and uneven sequencing depth. Moreover, the accuracy of scaffolding greatly depends on the quality of contigs. Generally, the existing scaffolding methods construct a scaffold graph, and then optimize the graph by deleting spurious edges. Nevertheless, due to the wrong joints between contigs, some correct edges connecting contigs may be deleted. Results In this study, we present a novel scaffolding method SCOP, which is the first method to classify the contigs and utilize the vertices and edges to optimize the scaffold graph. Specially, SCOP employs alignment features and GC-content of paired reads to evaluate the quality of contigs (vertices), and divide the contigs into three types (True, Uncertain and Misassembled), and then optimizes the scaffold graph based on the classification of contigs together with the alignment of edges. The experiment results on the datasets of GAGE-A and GAGE-B demonstrate that SCOP performs better than 12 other competing scaffolders. Availability and implementation SCOP is publicly available for download at https://github.com/bioinfomaticsCSU/SCOP. Supplementary information Supplementary data are available at Bioinformatics online." @default.
• W2890720538 created "2018-09-27" @default.
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• W2890720538 date "2018-09-03" @default.
• W2890720538 modified "2023-10-16" @default.
• W2890720538 title "SCOP: a novel scaffolding algorithm based on contig classification and optimization" @default.
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• W2890720538 doi "https://doi.org/10.1093/bioinformatics/bty773" @default.
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