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- W2890720796 abstract "Elucidation of intracellular signaling pathway is essential to understanding the regulatory mechanisms of cellular function. Mitogen-activated protein kinase (MAPK) signaling cascades are a major pathway in intracellular signaling. The methods used to detect cascade activation involve measurements of protein kinase activities. This unit describes an assay for specific immunoprecipitation of a protein kinase and detection of phosphorylation of the appropriate substrate, an in-gel kinase assay for kinases of unknown identity or for which there are no specific antibodies, and the JNK assay, which involves a broad-based immunoaffinity purification followed by phosphorylation." @default.
- W2890720796 created "2018-09-27" @default.
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- W2890720796 date "2004-07-01" @default.
- W2890720796 modified "2023-09-23" @default.
- W2890720796 title "Detection ofMAPKinase Signaling" @default.
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- W2890720796 doi "https://doi.org/10.1002/0471142727.mb1812s67" @default.
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