Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890767432> ?p ?o ?g. }
- W2890767432 abstract "CRISPR/Cas9 technology has revolutionized genome editing in mice, allowing for simple and rapid development of knockouts and knockins. CRISPR relies on small guide RNAs that direct the RNA-guided nuclease Cas9 to a designated genomic site using ∼20 bp of corresponding sequence. Cas9 then creates a double-strand break in the targeted loci that is either patched in an error-prone fashion to produce a frame-shift mutation, a knockout, or is repaired by recombination with donor DNA containing homology arms, a knockin. This protocol covers the techniques needed to rapidly generate knockout and knockin mice with CRISPR via microinjection of Cas9, the guide RNA, and possible donor DNA into the mouse zygote. © 2018 by John Wiley & Sons, Inc." @default.
- W2890767432 created "2018-09-27" @default.
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- W2890767432 creator A5078623302 @default.
- W2890767432 creator A5086788529 @default.
- W2890767432 date "2018-09-04" @default.
- W2890767432 modified "2023-09-26" @default.
- W2890767432 title "Genome Editing in Mice Using CRISPR/Cas9 Technology" @default.
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- W2890767432 doi "https://doi.org/10.1002/cpcb.57" @default.
- W2890767432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30178917" @default.
- W2890767432 hasPublicationYear "2018" @default.
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