Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890779976> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2890779976 endingPage "224" @default.
- W2890779976 startingPage "217" @default.
- W2890779976 abstract "Novel drugs possessing a mechanism of action specific to pathogenic mycobacteria, including Mycobacterium tuberculosis, are needed. In 2010, we discovered that the biosynthetic pathway of phosphatidylinositol, which is a membrane phospholipid, differs between humans and mycobacteria. The key enzyme responsible for this difference is phosphatidylinositol phosphate (PIP) synthase, which is present only in a few bacteria belonging to the phylum Actinobacteria. Discovering compounds that inhibit the activity of this enzyme will lead to the development of new drugs specific to pathogenic mycobacteria. Measuring PIP synthase activity requires the isotope-labeled substrate 1l-myo-inositol 1-phosphate (1l-Ino-1P). Because this substrate is not commercially available, we synthesized it from [14C] glucose 6-phosphate ([14C] Glc-6P), using a crude enzyme solution isolated from the methanoarchaeon 1l-Ino-1P synthase. The activity of 1l-Ino-1P synthase in the crude enzyme mixture was low, and quantitative analysis of the synthesized 1l-Ino-1P was inaccurate due to impurities present in the crude enzyme mixture. In the present study, we describe a method for synthesizing 1l-Ino-1P using a solution containing recombinant 1l-Ino-1P synthase derived from the hyperthermophilic archaeon Aeropyrum pernix. In addition, we elucidate the conditions leading to the almost complete conversion of Glc-6P into 1l-Ino-1P using this enzyme. Quantitation of the synthesized 1l -Ino-1P was performed by colorimetry and gas liquid chromatography. Further, we confirmed that isotope-labeled 1l-Ino-1P, which is difficult to quantitate by gas liquid chromatography, can be accurately quantified by colorimetry. We also confirmed that 1d-inositol 1-phosphate cannot be a substrate for PIP synthase." @default.
- W2890779976 created "2018-09-27" @default.
- W2890779976 creator A5008086780 @default.
- W2890779976 creator A5021732231 @default.
- W2890779976 creator A5049159053 @default.
- W2890779976 creator A5052694902 @default.
- W2890779976 creator A5079447828 @default.
- W2890779976 date "2018-09-01" @default.
- W2890779976 modified "2023-09-27" @default.
- W2890779976 title "Preparation of 1L-<i>myo-</i>Inositol 1-Phosphate as a Substrate of Phosphatidylinositol Phosphate Synthase" @default.
- W2890779976 cites W1513897103 @default.
- W2890779976 cites W1676948740 @default.
- W2890779976 cites W1965680002 @default.
- W2890779976 cites W1976113466 @default.
- W2890779976 cites W1980009517 @default.
- W2890779976 cites W2022882959 @default.
- W2890779976 cites W2055881650 @default.
- W2890779976 cites W2057994294 @default.
- W2890779976 cites W2060526875 @default.
- W2890779976 cites W2070788097 @default.
- W2890779976 cites W2109243443 @default.
- W2890779976 cites W2119225958 @default.
- W2890779976 cites W2140981202 @default.
- W2890779976 cites W2338750746 @default.
- W2890779976 doi "https://doi.org/10.7888/juoeh.40.217" @default.
- W2890779976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30224617" @default.
- W2890779976 hasPublicationYear "2018" @default.
- W2890779976 type Work @default.
- W2890779976 sameAs 2890779976 @default.
- W2890779976 citedByCount "1" @default.
- W2890779976 countsByYear W28907799762023 @default.
- W2890779976 crossrefType "journal-article" @default.
- W2890779976 hasAuthorship W2890779976A5008086780 @default.
- W2890779976 hasAuthorship W2890779976A5021732231 @default.
- W2890779976 hasAuthorship W2890779976A5049159053 @default.
- W2890779976 hasAuthorship W2890779976A5052694902 @default.
- W2890779976 hasAuthorship W2890779976A5079447828 @default.
- W2890779976 hasBestOaLocation W28907799761 @default.
- W2890779976 hasConcept C104317684 @default.
- W2890779976 hasConcept C112243037 @default.
- W2890779976 hasConcept C11960822 @default.
- W2890779976 hasConcept C170493617 @default.
- W2890779976 hasConcept C181199279 @default.
- W2890779976 hasConcept C185592680 @default.
- W2890779976 hasConcept C18903297 @default.
- W2890779976 hasConcept C2777132085 @default.
- W2890779976 hasConcept C2777289219 @default.
- W2890779976 hasConcept C2777427919 @default.
- W2890779976 hasConcept C2778169975 @default.
- W2890779976 hasConcept C2780610907 @default.
- W2890779976 hasConcept C42062724 @default.
- W2890779976 hasConcept C43617362 @default.
- W2890779976 hasConcept C523546767 @default.
- W2890779976 hasConcept C54355233 @default.
- W2890779976 hasConcept C55493867 @default.
- W2890779976 hasConcept C64299311 @default.
- W2890779976 hasConcept C86803240 @default.
- W2890779976 hasConceptScore W2890779976C104317684 @default.
- W2890779976 hasConceptScore W2890779976C112243037 @default.
- W2890779976 hasConceptScore W2890779976C11960822 @default.
- W2890779976 hasConceptScore W2890779976C170493617 @default.
- W2890779976 hasConceptScore W2890779976C181199279 @default.
- W2890779976 hasConceptScore W2890779976C185592680 @default.
- W2890779976 hasConceptScore W2890779976C18903297 @default.
- W2890779976 hasConceptScore W2890779976C2777132085 @default.
- W2890779976 hasConceptScore W2890779976C2777289219 @default.
- W2890779976 hasConceptScore W2890779976C2777427919 @default.
- W2890779976 hasConceptScore W2890779976C2778169975 @default.
- W2890779976 hasConceptScore W2890779976C2780610907 @default.
- W2890779976 hasConceptScore W2890779976C42062724 @default.
- W2890779976 hasConceptScore W2890779976C43617362 @default.
- W2890779976 hasConceptScore W2890779976C523546767 @default.
- W2890779976 hasConceptScore W2890779976C54355233 @default.
- W2890779976 hasConceptScore W2890779976C55493867 @default.
- W2890779976 hasConceptScore W2890779976C64299311 @default.
- W2890779976 hasConceptScore W2890779976C86803240 @default.
- W2890779976 hasIssue "3" @default.
- W2890779976 hasLocation W28907799761 @default.
- W2890779976 hasLocation W28907799762 @default.
- W2890779976 hasOpenAccess W2890779976 @default.
- W2890779976 hasPrimaryLocation W28907799761 @default.
- W2890779976 hasRelatedWork W1602874367 @default.
- W2890779976 hasRelatedWork W1723974516 @default.
- W2890779976 hasRelatedWork W1882082870 @default.
- W2890779976 hasRelatedWork W1976113466 @default.
- W2890779976 hasRelatedWork W1989744963 @default.
- W2890779976 hasRelatedWork W2007681847 @default.
- W2890779976 hasRelatedWork W2054525580 @default.
- W2890779976 hasRelatedWork W2171787368 @default.
- W2890779976 hasRelatedWork W2409223726 @default.
- W2890779976 hasRelatedWork W2890779976 @default.
- W2890779976 hasVolume "40" @default.
- W2890779976 isParatext "false" @default.
- W2890779976 isRetracted "false" @default.
- W2890779976 magId "2890779976" @default.
- W2890779976 workType "article" @default.