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• W2890785325 abstract "The formation of matured and individual sperm involves a series of molecular and spectacular morphological changes of the developing cysts in Drosophila melanogaster testis. Recent advances in RNA Sequencing (RNA-Seq) technology help us to understand the complexity of eukaryotic transcriptomes by dissecting different tissues and developmental stages of organisms. To gain a better understanding of cellular differentiation of spermatogenesis, we applied RNA-Seq to analyse the testis-specific transcriptome, including coding and non-coding genes.We isolated three different parts of the wild-type testis by dissecting and cutting the different regions: 1.) the apical region, which contains stem cells and developing spermatocytes 2.) the middle region, with enrichment of meiotic cysts 3.) the basal region, which contains elongated post-meiotic cysts with spermatids. Total RNA was isolated from each region and analysed by next-generation sequencing. We collected data from the annotated 17412 Drosophila genes and identified 5381 genes with significant transcript accumulation differences between the regions, representing the main stages of spermatogenesis. We demonstrated for the first time the presence and region specific distribution of 2061 lncRNAs in testis, with 203 significant differences. Using the available modENCODE RNA-Seq data, we determined the tissue specificity indices of Drosophila genes. Combining the indices with our results, we identified genes with region-specific enrichment in testis.By multiple analyses of our results and integrating existing knowledge about Drosophila melanogaster spermatogenesis to our dataset, we were able to describe transcript composition of different regions of Drosophila testis, including several stage-specific transcripts. We present searchable visualizations that can facilitate the identification of new components that play role in the organisation and composition of different stages of spermatogenesis, including the less known, but complex regulation of post-meiotic stages." @default.
• W2890785325 created "2018-09-27" @default.
• W2890785325 creator A5025266223 @default.
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• W2890785325 date "2018-09-24" @default.
• W2890785325 modified "2023-10-14" @default.
• W2890785325 title "Analysis of Drosophila melanogaster testis transcriptome" @default.
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• W2890785325 cites W1964193453 @default.
• W2890785325 cites W1966234416 @default.
• W2890785325 cites W1969561254 @default.
• W2890785325 cites W1978680540 @default.
• W2890785325 cites W1980300512 @default.
• W2890785325 cites W1980331623 @default.
• W2890785325 cites W1981093360 @default.
• W2890785325 cites W1981192346 @default.
• W2890785325 cites W1992123560 @default.
• W2890785325 cites W1996110479 @default.
• W2890785325 cites W2004843072 @default.
• W2890785325 cites W2006307886 @default.
• W2890785325 cites W2008289758 @default.
• W2890785325 cites W2008995997 @default.
• W2890785325 cites W2023577211 @default.
• W2890785325 cites W2027417565 @default.
• W2890785325 cites W2038963685 @default.
• W2890785325 cites W2045220576 @default.
• W2890785325 cites W2051101560 @default.
• W2890785325 cites W2058065156 @default.
• W2890785325 cites W2060245619 @default.
• W2890785325 cites W2061156149 @default.
• W2890785325 cites W2079999380 @default.
• W2890785325 cites W2084667612 @default.
• W2890785325 cites W2088544488 @default.
• W2890785325 cites W2092951535 @default.
• W2890785325 cites W2094100752 @default.
• W2890785325 cites W2100248019 @default.
• W2890785325 cites W2100256232 @default.
• W2890785325 cites W2104698675 @default.
• W2890785325 cites W2108626125 @default.
• W2890785325 cites W2109277926 @default.
• W2890785325 cites W2110287009 @default.
• W2890785325 cites W2113164383 @default.
• W2890785325 cites W2117107789 @default.
• W2890785325 cites W2120982603 @default.
• W2890785325 cites W2121818600 @default.
• W2890785325 cites W2124840547 @default.
• W2890785325 cites W2132836578 @default.
• W2890785325 cites W2139305477 @default.
• W2890785325 cites W2148090243 @default.
• W2890785325 cites W2148664612 @default.
• W2890785325 cites W2150123812 @default.
• W2890785325 cites W2151034383 @default.
• W2890785325 cites W2159675211 @default.
• W2890785325 cites W2160256652 @default.
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• W2890785325 cites W2167364244 @default.
• W2890785325 cites W2167496283 @default.
• W2890785325 cites W2168589268 @default.
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• W2890785325 cites W2205257234 @default.
• W2890785325 cites W2308929896 @default.
• W2890785325 cites W2321311605 @default.
• W2890785325 cites W2322466905 @default.
• W2890785325 cites W2418263429 @default.
• W2890785325 cites W2503531540 @default.
• W2890785325 cites W2512032906 @default.
• W2890785325 cites W2514088426 @default.
• W2890785325 cites W2516456939 @default.
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• W2890785325 cites W2538646406 @default.
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• W2890785325 cites W2741487225 @default.
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• W2890785325 doi "https://doi.org/10.1186/s12864-018-5085-z" @default.
• W2890785325 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6154878" @default.
• W2890785325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30249207" @default.
• W2890785325 hasPublicationYear "2018" @default.
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