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• W2890842959 abstract "Abstract Introduction Development of additional parameters for complete blood count has emerged in recent hematology analyzers, leading to many publications. However, few studies have been conducted on advanced RBC parameters and hemolytic anemias. We investigated the interest of Sysmex unique parameters, MicroR and HypoHe, as well as the immature fraction of reticulocytes ( IRF ) in combination with complete blood and reticulocyte count, for screening hereditary spherocytosis (HS) and pyruvate kinase deficiency. Methods We analyzed 182 samples using Sysmex XE ‐5000 analyzers from a cohort of red cell disorder patients from the Rouen University Hospital. These included 47 HS, 17 pyruvate kinase deficiencies, sickle cell diseases and trait, β‐thalassemia minor, iron deficiencies, and 489 samples from a routine group. Results Combining five parameters (hemoglobin level, reticulocyte count, IRF , MicroR, and %HypoHe), we developed a specific screening tool for HS allowing a sensitivity of 100% and a specificity of 92.1% and a specific screening tool for pyruvate kinase deficiencies allowing a sensitivity of 100% and a specificity of 96.5%. These parameters were also found accurate in infants and in HS without anemia. Conclusion We propose a costless, easy‐to‐use, and efficient approach to detect HS and pyruvate kinase deficiencies using Sysmex analyzers. These screening tools may help diagnosis of these disorders, help prevent complications, and result in a better management of these patients." @default.
• W2890842959 created "2018-09-27" @default.
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• W2890842959 date "2018-09-05" @default.
• W2890842959 modified "2023-09-27" @default.
• W2890842959 title "Screening of hereditary spherocytosis and pyruvate kinase deficiency by automated blood count using erythrocytic and reticulocytic parameters" @default.
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• W2890842959 doi "https://doi.org/10.1111/ijlh.12906" @default.
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