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- W2890865404 abstract "Duchenne muscular dystrophy (DMD) is a severe type of muscular dystrophy caused by mutation in the dystrophin gene and characterized by progressive muscle degeneration. One of the potential gene transfer agent for its treatment is an adeno-associated viral vectors (AAV). Utrophin, an autosomal homologue of dystrophin, can functionally compensate for the absence of dystrophin in DMD. Membrane expression of utrophin is detected in fetal myofibers but in adult mice it remains only in neuromuscular junctions. AAV-mediated delivery of utrophin to the mdx/utrn double-knockout mice showed improvements in dystrophic phenotype. The effect of exogenously delivered utrophin is not studied in mdx mouse model characterized by absence of dystrophin but not utrophin. The aim of this study was to control expression of AAV9-associated gene constructs after intramuscular administration and evaluate the effects of delivered agents on physiological parameters after intravenous administration of three types of microutrophin (human, human codon-optimized and murine) to adult mdx mice. To assess muscle function in mice we used hanging wire test and measured loss of force after lengthening contraction of hindlimb muscle. For expression verification and localization study we performed immunofluorescence analysis and western blotting. Two weeks after intramuscular administration in adult mice three types of microutrophins were detected in the sarcolemma. The maximum hanging time was 3-fold significantly increased five weeks after iv administration of human codon-optimized microutrophin and 2.5-fold increased after administration of murine microutrophin. Differences in force deficit after lengthening contraction and creatine kinase blood level were not detected. The most significant findings of this study was that AAV9-mediated delivery of human codon-optimized and murine microutrophin showed improvements in muscle strength of mdx mice." @default.
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- W2890865404 date "2018-10-01" @default.
- W2890865404 modified "2023-09-26" @default.
- W2890865404 title "DMD TREATMENT: ANIMAL MODELS" @default.
- W2890865404 doi "https://doi.org/10.1016/j.nmd.2018.06.247" @default.
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