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- W2890898213 abstract "<ns4:p>We previously published a method that infers chromosome conformation from images of fluorescently-tagged genomic loci, for the case when there are many loci labeled with each distinguishable color. Here we build on our previous work and improve the reconstruction algorithm to address previous limitations. We show that these improvements 1) increase the reconstruction accuracy and 2) allow the method to be used on large-scale problems involving several hundred labeled loci. Simulations indicate that full-chromosome reconstructions at 1/2 Mb resolution are possible using existing labeling and imaging technologies. The updated reconstruction code and the script files used for this paper are available at: <ns4:ext-link xmlns:ns3=http://www.w3.org/1999/xlink ext-link-type=uri ns3:href=https://github.com/heltilda/align3d>https://github.com/heltilda/align3d</ns4:ext-link>.</ns4:p>" @default.
- W2890898213 created "2018-09-27" @default.
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- W2890898213 date "2019-03-28" @default.
- W2890898213 modified "2023-09-25" @default.
- W2890898213 title "Improved inference of chromosome conformation from images of labeled loci" @default.
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- W2890898213 doi "https://doi.org/10.12688/f1000research.16252.3" @default.
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