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- W2890901760 abstract "The folding of many globular proteins from the unfolded (U) to the native (N) state appears to be describable by a two-state N ↔ U model, which has led to the general belief that protein folding occurs in a highly cooperative manner. One reason for the widespread belief in two-state folding is that protein folding reactions are invariably studied by ensemble averaging probes and not by probes that can distinguish as well as quantify the multiple conformations that may be present. Consequently, how cooperativity is affected by protein stability, protein sequence, and solvent conditions is poorly understood. In this study, hydrogen exchange coupled to mass spectrometry (HX-MS) of the PI3K SH3 domain was carried out in the presence of a stabilizing osmolyte, trimethylamine N-oxide (TMAO). By showing that HX occurs under the EX1 regime even in the presence of 2 M TMAO, we were able to examine the temporal evolution of the populations of the different conformations present together. A strong link between protein folding cooperativity and protein stability is revealed. Increasing stability is accompanied by an increase in the ruggedness of the free energy landscape as well as diminished cooperativity; the number of amide sites simultaneously opening up their structure decreases with an increase in TMAO concentration. A comparison of the effect of TMAO to that of urea on the intrinsic dynamics of the PI3K SH3 domain indicates that TMAO counteracts the effect of urea not only on protein stability but also on protein folding cooperativity." @default.
- W2890901760 created "2018-09-27" @default.
- W2890901760 creator A5041508571 @default.
- W2890901760 creator A5057549416 @default.
- W2890901760 date "2018-09-10" @default.
- W2890901760 modified "2023-09-24" @default.
- W2890901760 title "The Osmolyte TMAO Modulates Protein Folding Cooperativity by Altering Global Protein Stability" @default.
- W2890901760 cites W1879220584 @default.
- W2890901760 cites W1967147564 @default.
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- W2890901760 cites W1972862814 @default.
- W2890901760 cites W1973644706 @default.
- W2890901760 cites W1976179026 @default.
- W2890901760 cites W1976230062 @default.
- W2890901760 cites W1977179882 @default.
- W2890901760 cites W1985037445 @default.
- W2890901760 cites W1985716931 @default.
- W2890901760 cites W1986115510 @default.
- W2890901760 cites W1988949963 @default.
- W2890901760 cites W1989297730 @default.
- W2890901760 cites W1993650648 @default.
- W2890901760 cites W1996607243 @default.
- W2890901760 cites W1998266346 @default.
- W2890901760 cites W2004373243 @default.
- W2890901760 cites W2006588066 @default.
- W2890901760 cites W2006708449 @default.
- W2890901760 cites W2008818819 @default.
- W2890901760 cites W2012310626 @default.
- W2890901760 cites W2038816853 @default.
- W2890901760 cites W2043517534 @default.
- W2890901760 cites W2043750499 @default.
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- W2890901760 cites W2049662339 @default.
- W2890901760 cites W2050956052 @default.
- W2890901760 cites W2054056706 @default.
- W2890901760 cites W2054134770 @default.
- W2890901760 cites W2055595280 @default.
- W2890901760 cites W2060528711 @default.
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- W2890901760 cites W2096409300 @default.
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- W2890901760 doi "https://doi.org/10.1021/acs.biochem.8b00698" @default.
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