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• W2890910041 abstract "The present study was designed to find out the chemical composition of the essential oil of O.gratissimum Linn., grown in Tamilnadu by GC-MS method. Further isolation chemical constituents from aqueous extract of O.gratissimum Linn., and identified by IR, NMR and Mass spectroscopy methods. The anti-microbial activity of volatile oil and aqueous extract was checked against some micro-organisms. To the best of our knowledge, no such comparison studies have been reported earlier in the literature. Plan of work: Collection of the fresh leaves of O.gratissimum plant, Authentication of O.gratissimum leaves, Isolation of essential oils from O.gratissimum by using Clevenger apparatus with water as solvent., Determination of organoleptic characters, solubility and percentage yield of volatile oils,Measurement of following Physiochemical constants of essential oil of O.gratissimum leaves. i.e. Specific gravity, Optical rotation , Refractive index and Analysis of chemical composition of essential oil of O.gratissimum leaves by GC-MS analysis. Preparation of aqueous extract of O.gratissimum by different methods. Preliminary phytochemical studies of O.gratissimum Quantitative estimation of phytochemicals. Study of physico-chemical constant of O.gratissimum leaves aqueous extract. Qualitative analysis of inorganic compounds in O.gratissimum leaves. Quantitative analysis of inorganic compounds in O.gratissimum leaves Column chromatography of O.gratissimum aqueous extract. Identification of isolated compound by IR, NMR and Mass spectroscopy methods and Anti-microbial activity of volatile oil and aqueous extract of O.grattissimum leaves. Medicinal plants have played a vital role in treating diseases and in promoting health of mankind for a long time. They continue to become an important source of medicinal agents. The volatile oil of plant leaves was taken by Clevenger apparatus and the physical constants such as specific gravity, refractive index, optical rotation obtained confirms that the O.gratissimum oil is pure and posing the characteristics of a volatile oil. The total ion chromatogram retention time is about 20 min. Estragole, linalool and camphor was the major compounds. On the extraction of O.gratissimum leaves by different methods (mechanical, by soxhlet apparatus and by using microwave oven), the microwave oven method offered more compounds when compared with other methods. The leaves of studied plant contained accountable amount of phyto-chemicals and mineral contents. The anti-microbial activity of the O.gratissimum leaves oil and its aqueous extract having significant activity against tested bacterial and fungal strains when compared with standards ciprofloxacin (anti-bacterial) and ketaconazole (anti-fungal). The isolated compound Quercetin from column chromatography was identified by IR, NMR and Mass spectroscopy analysis and which may be responsible for the anti-microbial activity." @default.
• W2890910041 created "2018-09-27" @default.
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• W2890910041 date "2012-05-01" @default.
• W2890910041 modified "2023-09-24" @default.
• W2890910041 title "Chemical Profiling and Biological Activity on Leaves of Ocimum Gratissimum Grown in Tamilnadu." @default.
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