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• W2890923617 abstract "ABSTRACT The pathogenic spirochete Borrelia burgdorferi senses and responds to diverse environmental challenges, including changes in nutrient availability, throughout its enzootic cycle in Ixodes spp. ticks and vertebrate hosts. This study examined the role of DnaK suppressor protein (DksA) in the transcriptional response of B. burgdorferi to starvation. Wild-type and dksA mutant B. burgdorferi strains were subjected to starvation by shifting mid-logarithmic phase cultures grown in BSK II medium to serum-free RPMI medium for 6 h under microaerobic conditions (5% CO 2 , 3% O 2 ). Microarray analyses of wild-type B. burgdorferi revealed that genes encoding flagellar components, ribosomal proteins, and DNA replication machinery were downregulated in response to starvation. DksA mediated transcriptomic responses to starvation in B. burgdorferi as the dksA -deficient strain differentially expressed only 47 genes in response to starvation compared to the 500 genes differentially expressed in wild-type strains. Consistent with a role for DksA in the starvation response of B. burgdorferi , fewer CFUs were observed for dksA mutant after prolonged starvation in RPMI medium compared to wild-type B. burgdorferi . Transcriptomic analyses revealed a partial overlap between the DksA regulon and the regulon of Rel Bbu , the guanosine tetraphosphate and guanosine pentaphosphate [(p)ppGpp] synthetase that controls the stringent response; the DksA regulon also included many plasmid-borne genes. Additionally, the dksA mutant strain exhibited constitutively elevated (p)ppGpp levels compared to the wild-type strain, implying a regulatory relationship between DksA and (p)ppGpp. Together, these data indicate that DksA along with (p)ppGpp direct the stringent response to effect B. burgdorferi adaptation to its environment. IMPORTANCE The Lyme disease bacterium Borrelia burgdorferi must sense and respond to diverse environments as it cycles between its tick vectors and various vertebrate hosts. B. burgdorferi must withstand prolonged periods of starvation while it resides in unfed Ixodes ticks. In this study, the regulatory protein DksA is shown to play a pivotal role controlling the transcriptional responses of B. burgdorferi to starvation. The results of this study suggest that DksA gene regulatory activity impacts B. burgdorferi metabolism, virulence gene expression, and the ability of this bacterium to complete its natural life cycle." @default.
• W2890923617 created "2018-09-27" @default.
• W2890923617 creator A5000180802 @default.
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• W2890923617 date "2018-09-20" @default.
• W2890923617 modified "2023-09-23" @default.
• W2890923617 title "DksA controls the response of the Lyme disease spirochete Borrelia burgdorferi to starvation" @default.
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• W2890923617 cites W1971866858 @default.
• W2890923617 cites W1980131133 @default.
• W2890923617 cites W1982138514 @default.
• W2890923617 cites W1988711688 @default.
• W2890923617 cites W1995792027 @default.
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• W2890923617 cites W2013636685 @default.
• W2890923617 cites W2014148312 @default.
• W2890923617 cites W2014754473 @default.
• W2890923617 cites W2022020007 @default.
• W2890923617 cites W2023359102 @default.
• W2890923617 cites W2036154785 @default.
• W2890923617 cites W2039160511 @default.
• W2890923617 cites W2043454090 @default.
• W2890923617 cites W2079580196 @default.
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• W2890923617 cites W2093772258 @default.
• W2890923617 cites W2096065240 @default.
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• W2890923617 cites W2112984193 @default.
• W2890923617 cites W2114301703 @default.
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• W2890923617 cites W2118316271 @default.
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• W2890923617 cites W2125809659 @default.
• W2890923617 cites W2130913568 @default.
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• W2890923617 cites W2132522708 @default.
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• W2890923617 cites W2147006435 @default.
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• W2890923617 doi "https://doi.org/10.1101/421636" @default.
• W2890923617 hasPublicationYear "2018" @default.
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