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- W2890925876 abstract "Abstract Mapping-by-sequencing is a rapid method for identifying both natural as well as induced variations in the genome. However, it requires extensive bioinformatics expertise along with the computational infrastructure to analyze the sequencing data and these requirements have limited its widespread adoption. In the current study, we develop an easy to use tool, artMAP, to discover ethyl methanesulfonate (EMS) induced mutations in the Arabidopsis genome. The artMAP pipeline consists of well-established tools including TrimGalore, BWA, BEDTools, SAMtools, and SnpEff which were integrated in a Docker container. artMAP provides a graphical user interface and can be run on a regular laptop and desktop, thereby limiting the bioinformatics expertise required. artMAP can process input sequencing files generated from single or paired-end sequencing. The results of the analysis are presented in interactive graphs which display the annotation details of each mutation. Due to its ease of use, artMAP made the identification of EMS-induced mutations in Arabidopsis possible with only a few mouse click. The source code of artMAP is available on Github ( https://github.com/RihaLab/artMAP )." @default.
- W2890925876 created "2018-09-27" @default.
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- W2890925876 date "2018-09-12" @default.
- W2890925876 modified "2023-09-27" @default.
- W2890925876 title "artMAP: a user-friendly tool for mapping EMS-induced mutations in Arabidopsis" @default.
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- W2890925876 doi "https://doi.org/10.1101/414433" @default.
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