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- W2890927690 abstract "The bacteriophage P1 Cre/lox system has been utilized in diverse fungi for marker recycling and exchange, generation of targeted chromosome translocations, and targeted deletion of interstitial chromosome segments. Here we show the application of this tool in the wheat and maize pathogen, Fusarium graminearum. We explored three different ways to introduce Cre into strains with floxed genes, namely transformation with an episomal or integrative plasmid (pLC28), fusion of protoplasts of strains carrying floxed genes with strains expressing Cre by forcing heterokaryons, and crosses between strains with floxed genes and strains expressing Cre to isolate progeny in which the target genes had been deleted during the cross. We used this system for the construction of strains bearing auxotrophic markers that were generated by gene replacement with positively selectable markers followed by Cre-mediated marker excision. In addition, updated protocols for transformation and crosses for F. graminearum are provided. In combination, strains and tools developed here add to the arsenal of methods that can be used to carry out molecular genetics with F. graminearum." @default.
- W2890927690 created "2018-09-27" @default.
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- W2890927690 date "2018-01-01" @default.
- W2890927690 modified "2023-09-27" @default.
- W2890927690 title "Application of the Cre/lox System to Construct Auxotrophic Markers for Quantitative Genetic Analyses in Fusarium graminearum" @default.
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- W2890927690 cites W1531658444 @default.
- W2890927690 cites W1540563425 @default.
- W2890927690 cites W1549962797 @default.
- W2890927690 cites W1556784368 @default.
- W2890927690 cites W1590757915 @default.
- W2890927690 cites W1745643721 @default.
- W2890927690 cites W1838672802 @default.
- W2890927690 cites W1850569493 @default.
- W2890927690 cites W1883003611 @default.
- W2890927690 cites W1913334531 @default.
- W2890927690 cites W1967287827 @default.
- W2890927690 cites W1967423136 @default.
- W2890927690 cites W1983188063 @default.
- W2890927690 cites W1992752113 @default.
- W2890927690 cites W1994898104 @default.
- W2890927690 cites W1999690668 @default.
- W2890927690 cites W2000816393 @default.
- W2890927690 cites W2008884943 @default.
- W2890927690 cites W2012674191 @default.
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- W2890927690 doi "https://doi.org/10.1007/978-1-4939-8724-5_16" @default.
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