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• W2890939282 abstract "Abstract Cell-free translational systems based on cellular lysates optimized for in vitro protein synthesis have multiple applications both in basic and applied science, ranging from studies of translation regulation to cell-free production of proteins and ribosome-nascent chain complexes. In order to achieve both high activity and reproducibility in a translational system, it is essential that the ribosomes in the cellular lysate are enzymatically active. Here we demonstrate that genomic disruption of genes encoding ribosome inactivating factors – HPF in Bacillus subtilis and Stm1 in Saccharomyces cerevisiae – robustly improve the activities of bacterial and yeast translational systems. A possible next step in developing strains for production of even more efficient cell-free translation systems could be achieved by combining a genomic disruption of the ribosome hibernation machinery with inactivation of the genes responsible for proteolysis and RNA degradation." @default.
• W2890939282 created "2018-09-27" @default.
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• W2890939282 date "2018-09-22" @default.
• W2890939282 modified "2023-09-27" @default.
• W2890939282 title "Elimination of ribosome inactivating factors improves the efficiency of Bacillus subtilis and Saccharomyces cerevisiae cell-free translational systems" @default.
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• W2890939282 doi "https://doi.org/10.1101/424044" @default.
• W2890939282 hasPublicationYear "2018" @default.
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