Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890946686> ?p ?o ?g. }
- W2890946686 endingPage "125" @default.
- W2890946686 startingPage "115" @default.
- W2890946686 abstract "Vascular smooth muscle cells (VSMCs) play essential roles in regulating blood vessel form and function and they are required for vascular tissue regeneration. Multipotent adipose derived stromal cells (ASCs) can be differentiated into VSMC-like cells, which can be used as a potential VSMC source for the development of vascular tissue. However, the effects of cryopreservation on the differentiation of ASCs towards VSMCs are poorly studied to date. This study compared fresh ASCs (FA) vs. cryopreserved ASCs (CA) with respect to their differentiation potential towards VSMC-like cells. The expression of contractile VSMC markers (such as smoothelin) and cell contractility were investigated. It was found that VSMC-like cells derived from CA expressed smoothelin gene and protein at lower levels and showed compromised contractility in response to vasoconstrictors, when compared with those derived from FA. Moreover, it was demonstrated that this negative effect of cryopreservation could be mediated by MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways. Treatment of CA with MEK1/2-ERK1/2 activator or IFNγ neutralizing antibodies enhanced Smad2/3 phosphorylation and showed a rescue of the negative effect of cryopreservation on the differentiation of ASCs towards VSMC-like cells. These findings are important for defining approaches that may use cryopreserved ASCs for vascular tissue regeneration." @default.
- W2890946686 created "2018-09-27" @default.
- W2890946686 creator A5048135300 @default.
- W2890946686 creator A5058923571 @default.
- W2890946686 creator A5072211414 @default.
- W2890946686 date "2018-10-01" @default.
- W2890946686 modified "2023-09-26" @default.
- W2890946686 title "Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cells" @default.
- W2890946686 cites W130232318 @default.
- W2890946686 cites W1505584712 @default.
- W2890946686 cites W1521673926 @default.
- W2890946686 cites W1593059529 @default.
- W2890946686 cites W1968703634 @default.
- W2890946686 cites W1973042763 @default.
- W2890946686 cites W1984098071 @default.
- W2890946686 cites W1991384207 @default.
- W2890946686 cites W1996803044 @default.
- W2890946686 cites W2000547590 @default.
- W2890946686 cites W2001205524 @default.
- W2890946686 cites W2002907090 @default.
- W2890946686 cites W2014840129 @default.
- W2890946686 cites W2027031792 @default.
- W2890946686 cites W2033624423 @default.
- W2890946686 cites W2037299986 @default.
- W2890946686 cites W2041373058 @default.
- W2890946686 cites W2046156745 @default.
- W2890946686 cites W2047394124 @default.
- W2890946686 cites W2049892812 @default.
- W2890946686 cites W2055282652 @default.
- W2890946686 cites W2055928785 @default.
- W2890946686 cites W2061072494 @default.
- W2890946686 cites W2063736240 @default.
- W2890946686 cites W2074491029 @default.
- W2890946686 cites W2080416062 @default.
- W2890946686 cites W2080667675 @default.
- W2890946686 cites W2083156208 @default.
- W2890946686 cites W2100155649 @default.
- W2890946686 cites W2100221605 @default.
- W2890946686 cites W2101024920 @default.
- W2890946686 cites W2109637340 @default.
- W2890946686 cites W2112911718 @default.
- W2890946686 cites W2117903414 @default.
- W2890946686 cites W2118212786 @default.
- W2890946686 cites W2121822802 @default.
- W2890946686 cites W2121923529 @default.
- W2890946686 cites W2122328347 @default.
- W2890946686 cites W2123073085 @default.
- W2890946686 cites W2131074202 @default.
- W2890946686 cites W2133493757 @default.
- W2890946686 cites W2136331365 @default.
- W2890946686 cites W2143136051 @default.
- W2890946686 cites W2144824258 @default.
- W2890946686 cites W2146038929 @default.
- W2890946686 cites W2146703042 @default.
- W2890946686 cites W2149576023 @default.
- W2890946686 cites W2149648512 @default.
- W2890946686 cites W2155124532 @default.
- W2890946686 cites W2159152499 @default.
- W2890946686 cites W2172299613 @default.
- W2890946686 cites W2174012507 @default.
- W2890946686 cites W2269632789 @default.
- W2890946686 cites W2274924551 @default.
- W2890946686 cites W2325318720 @default.
- W2890946686 cites W2343127218 @default.
- W2890946686 cites W2410251279 @default.
- W2890946686 cites W2587279911 @default.
- W2890946686 cites W2590511631 @default.
- W2890946686 cites W2746731449 @default.
- W2890946686 cites W2765187038 @default.
- W2890946686 cites W593020771 @default.
- W2890946686 doi "https://doi.org/10.1016/j.scr.2018.09.005" @default.
- W2890946686 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30273905" @default.
- W2890946686 hasPublicationYear "2018" @default.
- W2890946686 type Work @default.
- W2890946686 sameAs 2890946686 @default.
- W2890946686 citedByCount "4" @default.
- W2890946686 countsByYear W28909466862019 @default.
- W2890946686 countsByYear W28909466862020 @default.
- W2890946686 countsByYear W28909466862022 @default.
- W2890946686 crossrefType "journal-article" @default.
- W2890946686 hasAuthorship W2890946686A5048135300 @default.
- W2890946686 hasAuthorship W2890946686A5058923571 @default.
- W2890946686 hasAuthorship W2890946686A5072211414 @default.
- W2890946686 hasBestOaLocation W28909466861 @default.
- W2890946686 hasConcept C134018914 @default.
- W2890946686 hasConcept C16930146 @default.
- W2890946686 hasConcept C179933525 @default.
- W2890946686 hasConcept C196843134 @default.
- W2890946686 hasConcept C198826908 @default.
- W2890946686 hasConcept C2779395532 @default.
- W2890946686 hasConcept C2992686903 @default.
- W2890946686 hasConcept C502942594 @default.
- W2890946686 hasConcept C86803240 @default.
- W2890946686 hasConcept C95444343 @default.
- W2890946686 hasConceptScore W2890946686C134018914 @default.
- W2890946686 hasConceptScore W2890946686C16930146 @default.
- W2890946686 hasConceptScore W2890946686C179933525 @default.
- W2890946686 hasConceptScore W2890946686C196843134 @default.