Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890947289> ?p ?o ?g. }
- W2890947289 abstract "Somatic embryogenesis (SE) results from the transition of differentiated plant somatic cells into embryogenic cells that requires the extensive reprogramming of the somatic cell transcriptome. Commonly, the SE-involved genes are identified by analyzing the heterogeneous population of explant cells and thus, it is necessary to validate the expression of the candidate genes in the cells that are competent for embryogenic transition. Here, we optimized and implemented the whole mount in situ hybridization (WISH) method (Dastidar et al. 2016; Bleckmann and Dresselhaus, 2016) in order to analyze the spatiotemporal localization of miRNAs (miR156, miR166, miR390, miR167) and mRNAs such as WOX5 and PHABULOSA-target of miR165/166 during the SE that is induced in Arabidopsis explants. This study presents a detailed step-by-step description of the WISH procedure in which DIG-labeled LNA and RNA probes were used to detect miRNAs and mRNAs, respectively. The usefulness of the WISH in the functional analysis of the SE-involved regulatory pathways is demonstrated and the advantages of this method are highlighted: (1) the ability to analyze intact non-sectioned plant tissue; (ii) the specificity of transcript detection; (iii) the detection of miRNA and (iv) a semi-quantitative assessment of the RNA abundance." @default.
- W2890947289 created "2018-09-27" @default.
- W2890947289 creator A5000973604 @default.
- W2890947289 creator A5010349405 @default.
- W2890947289 creator A5041649675 @default.
- W2890947289 creator A5062593985 @default.
- W2890947289 creator A5088068371 @default.
- W2890947289 date "2018-09-04" @default.
- W2890947289 modified "2023-10-13" @default.
- W2890947289 title "Whole Mount in situ Localization of miRNAs and mRNAs During Somatic Embryogenesis in Arabidopsis" @default.
- W2890947289 cites W1597430939 @default.
- W2890947289 cites W1604622048 @default.
- W2890947289 cites W1932970392 @default.
- W2890947289 cites W1965588439 @default.
- W2890947289 cites W1971028992 @default.
- W2890947289 cites W1975514719 @default.
- W2890947289 cites W1976554678 @default.
- W2890947289 cites W2000611318 @default.
- W2890947289 cites W2004418929 @default.
- W2890947289 cites W2009542077 @default.
- W2890947289 cites W2020640963 @default.
- W2890947289 cites W2024343928 @default.
- W2890947289 cites W2026381383 @default.
- W2890947289 cites W2026407800 @default.
- W2890947289 cites W2030989926 @default.
- W2890947289 cites W2034859327 @default.
- W2890947289 cites W2040456144 @default.
- W2890947289 cites W2041453482 @default.
- W2890947289 cites W2044285871 @default.
- W2890947289 cites W2044527120 @default.
- W2890947289 cites W2055350908 @default.
- W2890947289 cites W2072454585 @default.
- W2890947289 cites W2078132652 @default.
- W2890947289 cites W2094779186 @default.
- W2890947289 cites W2100760550 @default.
- W2890947289 cites W2109728684 @default.
- W2890947289 cites W2113048095 @default.
- W2890947289 cites W2118025002 @default.
- W2890947289 cites W2121138694 @default.
- W2890947289 cites W2129944920 @default.
- W2890947289 cites W2130132005 @default.
- W2890947289 cites W2140855778 @default.
- W2890947289 cites W2143633505 @default.
- W2890947289 cites W2162023519 @default.
- W2890947289 cites W2162038712 @default.
- W2890947289 cites W2162905664 @default.
- W2890947289 cites W2169913308 @default.
- W2890947289 cites W2182732817 @default.
- W2890947289 cites W2319416716 @default.
- W2890947289 cites W2338856133 @default.
- W2890947289 cites W2404827404 @default.
- W2890947289 cites W2500927640 @default.
- W2890947289 cites W2523024488 @default.
- W2890947289 cites W2529759531 @default.
- W2890947289 cites W2572307168 @default.
- W2890947289 cites W2580415007 @default.
- W2890947289 cites W2581535074 @default.
- W2890947289 cites W2593720737 @default.
- W2890947289 cites W2731146370 @default.
- W2890947289 cites W2754886210 @default.
- W2890947289 cites W2766580223 @default.
- W2890947289 cites W2772843696 @default.
- W2890947289 cites W2953166908 @default.
- W2890947289 cites W302668659 @default.
- W2890947289 cites W933912540 @default.
- W2890947289 doi "https://doi.org/10.3389/fpls.2018.01277" @default.
- W2890947289 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6131960" @default.
- W2890947289 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30233621" @default.
- W2890947289 hasPublicationYear "2018" @default.
- W2890947289 type Work @default.
- W2890947289 sameAs 2890947289 @default.
- W2890947289 citedByCount "12" @default.
- W2890947289 countsByYear W28909472892018 @default.
- W2890947289 countsByYear W28909472892019 @default.
- W2890947289 countsByYear W28909472892020 @default.
- W2890947289 countsByYear W28909472892021 @default.
- W2890947289 countsByYear W28909472892022 @default.
- W2890947289 crossrefType "journal-article" @default.
- W2890947289 hasAuthorship W2890947289A5000973604 @default.
- W2890947289 hasAuthorship W2890947289A5010349405 @default.
- W2890947289 hasAuthorship W2890947289A5041649675 @default.
- W2890947289 hasAuthorship W2890947289A5062593985 @default.
- W2890947289 hasAuthorship W2890947289A5088068371 @default.
- W2890947289 hasBestOaLocation W28909472891 @default.
- W2890947289 hasConcept C104317684 @default.
- W2890947289 hasConcept C134305767 @default.
- W2890947289 hasConcept C143065580 @default.
- W2890947289 hasConcept C144024400 @default.
- W2890947289 hasConcept C145059251 @default.
- W2890947289 hasConcept C149923435 @default.
- W2890947289 hasConcept C150194340 @default.
- W2890947289 hasConcept C162317418 @default.
- W2890947289 hasConcept C201468095 @default.
- W2890947289 hasConcept C2779491563 @default.
- W2890947289 hasConcept C2908647359 @default.
- W2890947289 hasConcept C54355233 @default.
- W2890947289 hasConcept C67705224 @default.
- W2890947289 hasConcept C70721500 @default.
- W2890947289 hasConcept C77255625 @default.
- W2890947289 hasConcept C81439078 @default.