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- W2890975128 endingPage "341" @default.
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- W2890975128 abstract "We developed a degradation assay based on fluorescent protein substrates that are efficiently recognized, unfolded, translocated, and hydrolyzed by the proteasome. The substrates consist of three components: a proteasome-binding tag, a folded domain, and an initiation region. All the components of the model substrate can be changed to modulate degradation, and the assay can be performed in parallel in 384-well plates. These properties allow the assay to be used to explore a wide range of experimental conditions and to screen proteasome modulators." @default.
- W2890975128 created "2018-09-27" @default.
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- W2890975128 date "2018-01-01" @default.
- W2890975128 modified "2023-09-27" @default.
- W2890975128 title "Scalable In Vitro Proteasome Activity Assay" @default.
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- W2890975128 doi "https://doi.org/10.1007/978-1-4939-8706-1_21" @default.
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