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• W2890976138 abstract "Abstract Introduction External quality assessment (EQA) is an essential tool for quality assurance of analytical testing processes of p210 BCR‐ABL1 transcripts by RT‐qPCR. As an EQA provider, the National Center for Clinical Laboratories organized an EQA scheme of p210 BCR‐ABL1 testing in China for the first time to identify existing problems and ensure the reliability of p210 BCR‐ABL1 testing. Methods Using armored RNA technology, we first constructed pACYC‐MS2‐p210 and CG recombinant plasmids and expressed p210 and CG armored RNAs, with packaging segments of p210 BCR‐ABL1 fusion gene (FG) and four common control gene (CG) transcripts. Using these armored RNAs, we prepared lyophilized p210 quality control (QC) sample panels and evaluated detection performance of participating laboratories in China. Results Of the 66 participating laboratories, great variation was found with coefficient of variation (CV%) of raw p210 BCR‐ABL1 results basically ranging from 60.0% to 100.0%. In 24 International Scale (IS) laboratories, the CV% of results decreased from 82.4% to 61.6%, and the percentage of laboratories within 2‐, 3‐, and 5‐fold of the median values increased from 78.2%, 87.0%, and 92.1% to 80.1%, 89.4%, and 97.2%, respectively, after conversion with a laboratory‐specific conversion factor (CF); however, poorly converted results were also observed in laboratories resulting from changed components of RT‐qPCR procedures. False‐negative and false‐positive results were also found in the EQA. Conclusions Various problems were found for p210 BCR‐ABL1 detection in the EQA. By solving the existing problems, the performance of p210 BCR‐ABL1 detection can be improved, ensuring robust laboratory diagnostic capacities in China." @default.
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• W2890976138 date "2018-09-11" @default.
• W2890976138 modified "2023-10-16" @default.
• W2890976138 title "External quality assessment of p210 BCR‐ABL1 transcript quantification by RT‐qPCR: Findings and recommendations" @default.
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• W2890976138 doi "https://doi.org/10.1111/ijlh.12919" @default.
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