Matches in SemOpenAlex for { <https://semopenalex.org/work/W2890985370> ?p ?o ?g. }
- W2890985370 abstract "ABSTRACT The trafficking of components within cilia, called intraflagellar transport (IFT), is powered by kinesin-2 and dynein-2 motors. Loss of function in any subunit of the heterotrimeric KIF3A/KIF3B/KAP kinesin-2 motor prevents ciliogenesis in mammalian cells and has hindered an understanding of how kinesin-2 motors function in IFT. We used a chemical-genetic approach to engineer an inhibitable KIF3A/KIF3B (i3A/i3B) kinesin-2 motor that is capable of rescuing WT motor function in Kif3a/Kif3b double-knockout cells. Inhibitor addition blocks ciliogenesis or, if added to ciliated cells, blocks IFT within two minutes, which leads to a complete loss of primary cilia within six hours. The kinesin-2 family members KIF3A/KIF3C and KIF17 cannot rescue ciliogenesis in Kif3a/Kif3b double-knockout cells nor delay the disassembly of full-formed cilia upon i3A/i3B inhibition. These data suggest that KIF3A/KIF3B/KAP is the sole and essential motor for cilia assembly and function in mammalian cells, indicating a species-specific adaptation of kinesin-2 motors for IFT function." @default.
- W2890985370 created "2018-09-27" @default.
- W2890985370 creator A5008103087 @default.
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- W2890985370 creator A5075010929 @default.
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- W2890985370 date "2018-09-05" @default.
- W2890985370 modified "2023-09-26" @default.
- W2890985370 title "Acute inhibition of heterotrimeric kinesin-2 function reveals mechanisms of intraflagellar transport in mammalian cilia" @default.
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- W2890985370 doi "https://doi.org/10.1101/409318" @default.
- W2890985370 hasPublicationYear "2018" @default.
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