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- W2890985691 abstract "Histology and immunohistochemistry are routine methods of analysis to visualize microscopic anatomy and localize proteins within biological tissue. In neuroscience, as well as a plethora of other scientific fields, these techniques are used. Immunohistochemistry can be done on slide mounted tissue or free-floating sections. Preparing slide-mounted samples is a time intensive process. The following protocol for a technique, called the Megabrain, reduced the time taken to cryosection and mount brain tissue by up to 90% by combining multiple brains into a single frozen block. Furthermore, this technique reduced variability seen between staining rounds, in a large histochemical study. The current technique has been optimized for using rodent brain tissue in downstream immunohistochemical analyses; however, it can be applied to different scientific fields that use cryosectioning." @default.
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- W2890985691 date "2018-09-18" @default.
- W2890985691 modified "2023-10-14" @default.
- W2890985691 title "Simultaneous Cryosectioning of Multiple Rodent Brains" @default.
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- W2890985691 doi "https://doi.org/10.3791/58513" @default.
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