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• W2891044954 abstract "Protein quantification is essential in a great variety of biochemical assays, yet the inherent systematic errors associated with the concentration determination of intrinsically disordered proteins (IDPs) using classical methods are hardly appreciated. Routinely used assays for protein quantification, such as the Bradford assay or ultraviolet absorbance at 280 nm, usually seriously misestimate the concentrations of IDPs due to their distinct and variable amino acid composition. Therefore, dependable method(s) have to be worked out/adopted for this task. By comparison to elemental analysis as the gold standard, we show through the example of four globular proteins and nine IDPs that the ninhydrin assay and the commercial QubitTM Protein Assay provide reliable data on IDP quantity, but even these techniques are only recommended to use for IDPs following standardization. The far-reaching implications of this simple observation are demonstrated through two examples i) circular dichroism spectrum deconvolution, and ii) receptor-ligand affinity determination. These actual comparative examples illustrate the potential errors that can be incorporated into the biophysical parameters of IDPs, due to systematic misestimation of their concentration. This leads to inaccurate description of IDP functions." @default.
• W2891044954 created "2018-09-27" @default.
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• W2891044954 date "2018-09-04" @default.
• W2891044954 modified "2023-10-15" @default.
• W2891044954 title "Quantification of Intrinsically Disordered Proteins: A Problem Not Fully Appreciated" @default.
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• W2891044954 doi "https://doi.org/10.3389/fmolb.2018.00083" @default.
• W2891044954 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6131523" @default.
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