FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891070149> ?p ?o ?g. }

• W2891070149 endingPage "2199" @default.
• W2891070149 startingPage "2176" @default.
• W2891070149 abstract "Transcriptomic changes induced in one cell type by another mediate many biological processes in the brain and elsewhere; however, achieving artifact-free physical separation of cell types to study them is challenging and generally allows for analysis of only a single cell type. We describe an approach using a co-culture of distinct cell types from different species that enables physical cell sorting to be replaced by in silico RNA sequencing (RNA-seq) read sorting, which is possible because of evolutionary divergence of messenger RNA (mRNA) sequences. As an exemplary experiment, we describe the co-culture of purified neurons, astrocytes, and microglia from different species (12–14 d). We describe how to use our Python tool, Sargasso, to separate the reads from conventional RNA-seq according to species and to eliminate any artifacts borne of imperfect genome annotation (10 h). We show how this procedure, which requires no special skills beyond those that might normally be expected of wet lab and bioinformatics researchers, enables the simultaneous transcriptomic profiling of different cell types, revealing the distinct influence of microglia on astrocytic and neuronal transcriptomes under inflammatory conditions. This protocol describes the co-culture of cells from multiple species and, after RNA-seq, the separation of reads by species via the Sargasso bioinformatics pipeline to elucidate the effects of one cell type on the transcriptome of the others." @default.
• W2891070149 created "2018-09-27" @default.
• W2891070149 creator A5006025723 @default.
• W2891070149 creator A5014681613 @default.
• W2891070149 creator A5020254324 @default.
• W2891070149 creator A5044428518 @default.
• W2891070149 creator A5057638577 @default.
• W2891070149 creator A5061650623 @default.
• W2891070149 creator A5084953972 @default.
• W2891070149 date "2018-09-24" @default.
• W2891070149 modified "2023-09-27" @default.
• W2891070149 title "Mixed-species RNA-seq for elucidation of non-cell-autonomous control of gene transcription" @default.
• W2891070149 cites W1823204572 @default.
• W2891070149 cites W1849415743 @default.
• W2891070149 cites W1967368719 @default.
• W2891070149 cites W1972942992 @default.
• W2891070149 cites W1975822780 @default.
• W2891070149 cites W1977213877 @default.
• W2891070149 cites W1980775054 @default.
• W2891070149 cites W1995618392 @default.
• W2891070149 cites W2001188679 @default.
• W2891070149 cites W2008348489 @default.
• W2891070149 cites W2009307312 @default.
• W2891070149 cites W2012935976 @default.
• W2891070149 cites W2020509501 @default.
• W2891070149 cites W2021141360 @default.
• W2891070149 cites W2021880506 @default.
• W2891070149 cites W2049197565 @default.
• W2891070149 cites W2059996790 @default.
• W2891070149 cites W2062167746 @default.
• W2891070149 cites W2069498125 @default.
• W2891070149 cites W2097323692 @default.
• W2891070149 cites W2114104545 @default.
• W2891070149 cites W2115155255 @default.
• W2891070149 cites W2115565454 @default.
• W2891070149 cites W2119137351 @default.
• W2891070149 cites W2121964616 @default.
• W2891070149 cites W2122666578 @default.
• W2891070149 cites W2128505445 @default.
• W2891070149 cites W2129435019 @default.
• W2891070149 cites W2133259993 @default.
• W2891070149 cites W2138207763 @default.
• W2891070149 cites W2151003930 @default.
• W2891070149 cites W2159351939 @default.
• W2891070149 cites W2161104189 @default.
• W2891070149 cites W2168888447 @default.
• W2891070149 cites W2169456326 @default.
• W2891070149 cites W2179438025 @default.
• W2891070149 cites W2197124664 @default.
• W2891070149 cites W2234053937 @default.
• W2891070149 cites W2471976579 @default.
• W2891070149 cites W2527088361 @default.
• W2891070149 cites W2553837355 @default.
• W2891070149 cites W2572710398 @default.
• W2891070149 cites W2582141810 @default.
• W2891070149 cites W2590949976 @default.
• W2891070149 cites W2592811885 @default.
• W2891070149 cites W2604308930 @default.
• W2891070149 cites W2611496024 @default.
• W2891070149 cites W2753593979 @default.
• W2891070149 cites W4252232449 @default.
• W2891070149 doi "https://doi.org/10.1038/s41596-018-0029-2" @default.
• W2891070149 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30250293" @default.
• W2891070149 hasPublicationYear "2018" @default.
• W2891070149 type Work @default.
• W2891070149 sameAs 2891070149 @default.
• W2891070149 citedByCount "20" @default.
• W2891070149 countsByYear W28910701492019 @default.
• W2891070149 countsByYear W28910701492021 @default.
• W2891070149 countsByYear W28910701492022 @default.
• W2891070149 countsByYear W28910701492023 @default.
• W2891070149 crossrefType "journal-article" @default.
• W2891070149 hasAuthorship W2891070149A5006025723 @default.
• W2891070149 hasAuthorship W2891070149A5014681613 @default.
• W2891070149 hasAuthorship W2891070149A5020254324 @default.
• W2891070149 hasAuthorship W2891070149A5044428518 @default.
• W2891070149 hasAuthorship W2891070149A5057638577 @default.
• W2891070149 hasAuthorship W2891070149A5061650623 @default.
• W2891070149 hasAuthorship W2891070149A5084953972 @default.
• W2891070149 hasBestOaLocation W28910701492 @default.
• W2891070149 hasConcept C104317684 @default.
• W2891070149 hasConcept C107397762 @default.
• W2891070149 hasConcept C141231307 @default.
• W2891070149 hasConcept C1491633281 @default.
• W2891070149 hasConcept C150194340 @default.
• W2891070149 hasConcept C162317418 @default.
• W2891070149 hasConcept C189014844 @default.
• W2891070149 hasConcept C2775905019 @default.
• W2891070149 hasConcept C2778630268 @default.
• W2891070149 hasConcept C54355233 @default.
• W2891070149 hasConcept C67705224 @default.
• W2891070149 hasConcept C70721500 @default.
• W2891070149 hasConcept C86803240 @default.
• W2891070149 hasConceptScore W2891070149C104317684 @default.
• W2891070149 hasConceptScore W2891070149C107397762 @default.
• W2891070149 hasConceptScore W2891070149C141231307 @default.
• W2891070149 hasConceptScore W2891070149C1491633281 @default.
• W2891070149 hasConceptScore W2891070149C150194340 @default.

• next