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- W2891100575 abstract "Radix Dipsaci is employed for treating bone fractures in traditional Chinese medicine. In the current, a quantitative approach was established for determination of ten bioactive components (loganic acid, loganin, 3,5-diCQA, 4-CQA, 3,4-diCQA, sweroside, dipsacussaponin B, dipsacoside A, asperosaponin V and asperosaponin VI) in raw and rice wine-sautéed Radix Dipsaci by using UPLC-Q-TOF-MS based approach. The sample handling procedure was optimized. Chromatographic separation was operated on a Thermo Syncronis AQ-C18 UPLC column with mobile phase consisting of 0.01% aqueous formic acid and acetonitrile. The method was validated in terms of linearity, precision and recovery tests. All calibration curves displayed good linearity (R2 > 0.9991). The limit of detection and limit of quantification of these components were from 0.007 to 0.032 μg/mL and from 0.020 to 0.095 μg/mL, respectively. The average recoveries of these components were from 96.7 to 102.7% with relative standard deviation values from 0.13 to 3.81% for Radix Dipsaci. The developed approach can be employed for quality control of raw and wine-sautéed Radix Dipsaci." @default.
- W2891100575 created "2018-09-27" @default.
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- W2891100575 date "2018-09-21" @default.
- W2891100575 modified "2023-10-17" @default.
- W2891100575 title "Simultaneous Determination of Ten Bioactive Components in Raw and Processed Radix<i>Dipsaci</i>by UPLC-Q-TOF-MS" @default.
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- W2891100575 doi "https://doi.org/10.1093/chromsci/bmy093" @default.
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