Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891184187> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2891184187 abstract "ABSTRACT Multiplexing and absolute quantification of nucleic acids, both have, in their own right, significant and extensive use in biomedical related fields, especially in point-of-care applications. Currently, the ability to detect several nucleic acid targets in a single-reaction scales linearly with the number of targets; an expensive and time-consuming feat. Here, we propose a new methodology based on multidimensional standard curves that extends the use of real-time PCR data obtained by common qPCR instruments. By applying this novel methodology, we achieve simultaneous single-channel multiplexing and enhanced quantification of multiple targets using only real-time amplification data. This is obtained without the need of fluorescent probes, agarose gels, melting curves or sequencing analysis. Given the importance and demand for tackling challenges in antimicrobial resistance, the proposed method is applied to the four most prominent carbapenem-resistant genes: bla OXA-48 , bla NDM , bla VIM and bla KPC , which account for 97% of the UK’s reported carbapenemase-producing Enterobacteriaceae." @default.
- W2891184187 created "2018-09-27" @default.
- W2891184187 creator A5011294641 @default.
- W2891184187 creator A5038060499 @default.
- W2891184187 creator A5039531510 @default.
- W2891184187 creator A5044991598 @default.
- W2891184187 creator A5058525592 @default.
- W2891184187 creator A5069244062 @default.
- W2891184187 creator A5074759907 @default.
- W2891184187 date "2018-09-07" @default.
- W2891184187 modified "2023-09-26" @default.
- W2891184187 title "Simultaneous single-channel multiplex and quantification of carbapenem-resistant genes using multidimensional standard curves" @default.
- W2891184187 cites W2087828931 @default.
- W2891184187 cites W2106072567 @default.
- W2891184187 cites W2141766288 @default.
- W2891184187 cites W2156457897 @default.
- W2891184187 cites W2170994550 @default.
- W2891184187 cites W2513238037 @default.
- W2891184187 cites W2529931137 @default.
- W2891184187 cites W2759877444 @default.
- W2891184187 cites W2791995217 @default.
- W2891184187 cites W2884134784 @default.
- W2891184187 cites W4251707891 @default.
- W2891184187 doi "https://doi.org/10.1101/409912" @default.
- W2891184187 hasPublicationYear "2018" @default.
- W2891184187 type Work @default.
- W2891184187 sameAs 2891184187 @default.
- W2891184187 citedByCount "0" @default.
- W2891184187 crossrefType "posted-content" @default.
- W2891184187 hasAuthorship W2891184187A5011294641 @default.
- W2891184187 hasAuthorship W2891184187A5038060499 @default.
- W2891184187 hasAuthorship W2891184187A5039531510 @default.
- W2891184187 hasAuthorship W2891184187A5044991598 @default.
- W2891184187 hasAuthorship W2891184187A5058525592 @default.
- W2891184187 hasAuthorship W2891184187A5069244062 @default.
- W2891184187 hasAuthorship W2891184187A5074759907 @default.
- W2891184187 hasBestOaLocation W28911841871 @default.
- W2891184187 hasConcept C127162648 @default.
- W2891184187 hasConcept C19275194 @default.
- W2891184187 hasConcept C24107716 @default.
- W2891184187 hasConcept C2781188995 @default.
- W2891184187 hasConcept C31258907 @default.
- W2891184187 hasConcept C41008148 @default.
- W2891184187 hasConcept C54355233 @default.
- W2891184187 hasConcept C70721500 @default.
- W2891184187 hasConcept C76155785 @default.
- W2891184187 hasConcept C86803240 @default.
- W2891184187 hasConceptScore W2891184187C127162648 @default.
- W2891184187 hasConceptScore W2891184187C19275194 @default.
- W2891184187 hasConceptScore W2891184187C24107716 @default.
- W2891184187 hasConceptScore W2891184187C2781188995 @default.
- W2891184187 hasConceptScore W2891184187C31258907 @default.
- W2891184187 hasConceptScore W2891184187C41008148 @default.
- W2891184187 hasConceptScore W2891184187C54355233 @default.
- W2891184187 hasConceptScore W2891184187C70721500 @default.
- W2891184187 hasConceptScore W2891184187C76155785 @default.
- W2891184187 hasConceptScore W2891184187C86803240 @default.
- W2891184187 hasLocation W28911841871 @default.
- W2891184187 hasLocation W28911841872 @default.
- W2891184187 hasOpenAccess W2891184187 @default.
- W2891184187 hasPrimaryLocation W28911841871 @default.
- W2891184187 hasRelatedWork W2116490593 @default.
- W2891184187 hasRelatedWork W2148103276 @default.
- W2891184187 hasRelatedWork W2617507009 @default.
- W2891184187 hasRelatedWork W2775620371 @default.
- W2891184187 hasRelatedWork W2955498613 @default.
- W2891184187 hasRelatedWork W3182278458 @default.
- W2891184187 hasRelatedWork W4280654000 @default.
- W2891184187 hasRelatedWork W4285192699 @default.
- W2891184187 hasRelatedWork W4311391842 @default.
- W2891184187 hasRelatedWork W2085169680 @default.
- W2891184187 isParatext "false" @default.
- W2891184187 isRetracted "false" @default.
- W2891184187 magId "2891184187" @default.
- W2891184187 workType "article" @default.