FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891194007> ?p ?o ?g. }

• W2891194007 abstract "Abstract The retinotectal synapse in larval zebrafish, combined with live time-lapse imaging, provides an advantageous model for study of the development and remodelling of central synapses in vivo . In previous studies, these synapses were labelled by transient expression of fluorescence-tagged synaptic proteins, which resulted in the dramatic variation of labelling patterns in each larva. Here, using GAL4-Upstream Activating Sequence (GAL4-UAS) methodology, we generated stable transgenic lines, which express EGFP-tagged synaptophysin (a presynaptic protein) in retinal ganglion cells (RGCs), to reliably label the pre-synaptic site of retinotectal synapses. This tool avoids the variable labelling of RGCs that occurs in transient transgenic larvae. We obtained several stable transgenic lines that differ consistently in the number of labelled RGCs. Using stable lines that consistently had a single labelled RGC, we could trace synaptogenic dynamics on an individual RGC axonal arbor across different developmental stages. In the stable lines that consistently had multiple labelled RGCs, we could simultaneously monitor both pre- and post-synaptic compartments by combining transient labelling of post-synaptic sites on individual tectal neurons. These tools allowed us to investigate molecular events underlying synaptogenesis and found that the microRNA-132 ( miR-132 ) is required for developmental synaptogenesis. Thus, these transgenic zebrafish stable lines provide appropriate tools for studying central synaptogenesis and underlying molecular mechanisms in intact vertebrate brain." @default.
• W2891194007 created "2018-09-27" @default.
• W2891194007 creator A5000095180 @default.
• W2891194007 creator A5030968374 @default.
• W2891194007 creator A5066169613 @default.
• W2891194007 creator A5068400813 @default.
• W2891194007 creator A5071773009 @default.
• W2891194007 date "2018-09-19" @default.
• W2891194007 modified "2023-10-10" @default.
• W2891194007 title "A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis" @default.
• W2891194007 cites W1966155655 @default.
• W2891194007 cites W1972259766 @default.
• W2891194007 cites W1973643970 @default.
• W2891194007 cites W1974411081 @default.
• W2891194007 cites W1975437957 @default.
• W2891194007 cites W1977756287 @default.
• W2891194007 cites W1979019425 @default.
• W2891194007 cites W1979606683 @default.
• W2891194007 cites W1981186391 @default.
• W2891194007 cites W1983529672 @default.
• W2891194007 cites W2000430854 @default.
• W2891194007 cites W2005889545 @default.
• W2891194007 cites W2018570986 @default.
• W2891194007 cites W2019751519 @default.
• W2891194007 cites W2022062121 @default.
• W2891194007 cites W2025448194 @default.
• W2891194007 cites W2035145219 @default.
• W2891194007 cites W2038757483 @default.
• W2891194007 cites W2039243670 @default.
• W2891194007 cites W2043351945 @default.
• W2891194007 cites W2043972280 @default.
• W2891194007 cites W2045931260 @default.
• W2891194007 cites W2053850015 @default.
• W2891194007 cites W2060915232 @default.
• W2891194007 cites W2062045776 @default.
• W2891194007 cites W2062559387 @default.
• W2891194007 cites W2067829194 @default.
• W2891194007 cites W2074623278 @default.
• W2891194007 cites W2075371471 @default.
• W2891194007 cites W2082251914 @default.
• W2891194007 cites W2083246499 @default.
• W2891194007 cites W2084758662 @default.
• W2891194007 cites W2086536594 @default.
• W2891194007 cites W2088574135 @default.
• W2891194007 cites W2089233932 @default.
• W2891194007 cites W2090183355 @default.
• W2891194007 cites W2115109165 @default.
• W2891194007 cites W2117391623 @default.
• W2891194007 cites W2125430316 @default.
• W2891194007 cites W2126672460 @default.
• W2891194007 cites W2133093591 @default.
• W2891194007 cites W2143289847 @default.
• W2891194007 cites W2145961463 @default.
• W2891194007 cites W2149257146 @default.
• W2891194007 cites W2152284832 @default.
• W2891194007 cites W2162407586 @default.
• W2891194007 cites W2162983737 @default.
• W2891194007 cites W2293360927 @default.
• W2891194007 cites W2460789311 @default.
• W2891194007 cites W2491160165 @default.
• W2891194007 cites W2514163892 @default.
• W2891194007 cites W2568039432 @default.
• W2891194007 cites W2606039495 @default.
• W2891194007 cites W4237011200 @default.
• W2891194007 doi "https://doi.org/10.1038/s41598-018-32409-y" @default.
• W2891194007 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6145912" @default.
• W2891194007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30232367" @default.
• W2891194007 hasPublicationYear "2018" @default.
• W2891194007 type Work @default.
• W2891194007 sameAs 2891194007 @default.
• W2891194007 citedByCount "7" @default.
• W2891194007 countsByYear W28911940072020 @default.
• W2891194007 countsByYear W28911940072021 @default.
• W2891194007 countsByYear W28911940072022 @default.
• W2891194007 countsByYear W28911940072023 @default.
• W2891194007 crossrefType "journal-article" @default.
• W2891194007 hasAuthorship W2891194007A5000095180 @default.
• W2891194007 hasAuthorship W2891194007A5030968374 @default.
• W2891194007 hasAuthorship W2891194007A5066169613 @default.
• W2891194007 hasAuthorship W2891194007A5068400813 @default.
• W2891194007 hasAuthorship W2891194007A5071773009 @default.
• W2891194007 hasBestOaLocation W28911940071 @default.
• W2891194007 hasConcept C102230213 @default.
• W2891194007 hasConcept C104317684 @default.
• W2891194007 hasConcept C127445978 @default.
• W2891194007 hasConcept C141035611 @default.
• W2891194007 hasConcept C148738053 @default.
• W2891194007 hasConcept C169760540 @default.
• W2891194007 hasConcept C203014093 @default.
• W2891194007 hasConcept C204232928 @default.
• W2891194007 hasConcept C2776878037 @default.
• W2891194007 hasConcept C2777209535 @default.
• W2891194007 hasConcept C2778994924 @default.
• W2891194007 hasConcept C54355233 @default.
• W2891194007 hasConcept C65232495 @default.
• W2891194007 hasConcept C86803240 @default.
• W2891194007 hasConcept C95444343 @default.
• W2891194007 hasConceptScore W2891194007C102230213 @default.
• W2891194007 hasConceptScore W2891194007C104317684 @default.
• W2891194007 hasConceptScore W2891194007C127445978 @default.

• next