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- W2891220007 abstract "Highly porous chitosan microspheres (CSM) were prepared through emulsion-based thermally induced phase separation (TIPS) without using toxic crosslinkers and chemical porogenic agents other than ice. The CSM had an average diameter of ∼150 μm with interconnected pores varying from 20∼50 μm in size. Due to their excellent biocompatibility and unique porous structure, high-performance hepatocyte culture in three-dimensional (3D) space was achieved using the CSM as microcarriers, as cell growth also took place within the internal pores of the CSM, besides their external surface, and multidirectional cell-cell interactions were observed. Enhanced cellular activity and functions were obtained with the CSM microcarriers as compared with 2D cell culture. It is believed that these CSM microcarriers provide a promising platform for 3D cell culture in vitro." @default.
- W2891220007 created "2018-09-27" @default.
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- W2891220007 date "2018-12-01" @default.
- W2891220007 modified "2023-10-14" @default.
- W2891220007 title "Porous chitosan microspheres as microcarriers for 3D cell culture" @default.
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- W2891220007 doi "https://doi.org/10.1016/j.carbpol.2018.09.021" @default.
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