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- W2891242263 abstract "Abstract Combining luminescent transition metal complex with super‐resolution microscopy is an excellent strategy for the long‐term visualization of the dynamics of subcellular structures in living cells. However, it remains unclear whether iridium(III) complexes are applicable for a particular type of super‐resolution technique, structured illumination microscopy (SIM), to image subcellular structures. Herein, an iridium(III) dye, to track mitochondrial dynamics in living cells under SIM is described. The dye demonstrates excellent specificity and photostability and satisfactory cell permeability. While using SIM to image mitochondria, an ≈80 nm resolution is achieved that allows the clear observation of the structure of mitochondrial cristae. The dye is used to monitor and quantify mitochondrial dynamics relative to lysosomes, including fusion involved in mitophagy, and newly discovered mitochondria–lysosome contact (MLC) under different conditions. The MLC remains intact and fusion vanishes when five receptors, p62, NDP52, OPTN, NBR1, and TAX1BP1, are knocked out, suggesting that these two processes are independent." @default.
- W2891242263 created "2018-09-27" @default.
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- W2891242263 date "2018-09-14" @default.
- W2891242263 modified "2023-10-15" @default.
- W2891242263 title "Super‐Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore" @default.
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- W2891242263 doi "https://doi.org/10.1002/smll.201802166" @default.
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