Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891246271> ?p ?o ?g. }
- W2891246271 endingPage "31" @default.
- W2891246271 startingPage "27" @default.
- W2891246271 abstract "The availability of purified antibodies is a prerequisite for many applications and the appropriate choice(s) for antibody-purification is crucial. Numerous methods have been developed for the purification of antibodies from different sources with affinity chromatography-based methods being the most extensively utilized. These methods are based on high specificity, easy reversibility and biological interactions between two molecules (e.g., between receptor and ligand or antibody and antigen). However, no simple techniques have yet been described to characterize and purify subclasses of immunoglobulins (Ig) from some animals of biotechnology importance such as equines, which are frequently used to produce biotherapeutic antibodies. The sera of these animals present a large number of Ig classes that have a greater complexity than other animals. The implementation of an effective protocol to purify the desired antibody class/subclasses requires meticulous planning to achieve yields at a high purity. The IgG3 subclass of equine-Ig has recently been used as antigen in a new diagnostic test for allergic responses to horse sera-based therapies. Here, we defined a simple method using Jacalin lectin immobilized on Sepharose beads to prepare highly pure equine IgG3 antibodies with a determination of the affinity constants for Jacalin lectin and horse IgG3." @default.
- W2891246271 created "2018-09-27" @default.
- W2891246271 creator A5000219705 @default.
- W2891246271 creator A5000391285 @default.
- W2891246271 creator A5000706755 @default.
- W2891246271 creator A5002728543 @default.
- W2891246271 creator A5034131186 @default.
- W2891246271 creator A5047392255 @default.
- W2891246271 creator A5061896931 @default.
- W2891246271 creator A5071361779 @default.
- W2891246271 date "2018-11-01" @default.
- W2891246271 modified "2023-09-26" @default.
- W2891246271 title "Purification of equine IgG3 by lectin affinity and an interaction analysis via microscale thermophoresis" @default.
- W2891246271 cites W100223012 @default.
- W2891246271 cites W1572573094 @default.
- W2891246271 cites W1596232811 @default.
- W2891246271 cites W1992240357 @default.
- W2891246271 cites W1993129253 @default.
- W2891246271 cites W2000518549 @default.
- W2891246271 cites W2001927093 @default.
- W2891246271 cites W2003449739 @default.
- W2891246271 cites W2004014858 @default.
- W2891246271 cites W2005400112 @default.
- W2891246271 cites W2023820553 @default.
- W2891246271 cites W2056504172 @default.
- W2891246271 cites W2060386111 @default.
- W2891246271 cites W2065239602 @default.
- W2891246271 cites W2065823415 @default.
- W2891246271 cites W2065883855 @default.
- W2891246271 cites W2066060629 @default.
- W2891246271 cites W2069575004 @default.
- W2891246271 cites W2071040943 @default.
- W2891246271 cites W2083301609 @default.
- W2891246271 cites W2083571658 @default.
- W2891246271 cites W2086242039 @default.
- W2891246271 cites W2088487477 @default.
- W2891246271 cites W2093230062 @default.
- W2891246271 cites W2096563716 @default.
- W2891246271 cites W2100837269 @default.
- W2891246271 cites W2112516644 @default.
- W2891246271 cites W2114370846 @default.
- W2891246271 cites W2135885240 @default.
- W2891246271 cites W2142252167 @default.
- W2891246271 cites W2145971399 @default.
- W2891246271 cites W2159702923 @default.
- W2891246271 cites W2166721734 @default.
- W2891246271 cites W2256138902 @default.
- W2891246271 cites W2318264742 @default.
- W2891246271 cites W2341626671 @default.
- W2891246271 cites W2403226105 @default.
- W2891246271 cites W2413408814 @default.
- W2891246271 cites W2745744452 @default.
- W2891246271 cites W2754439863 @default.
- W2891246271 cites W2789356676 @default.
- W2891246271 cites W2789371552 @default.
- W2891246271 cites W2793163389 @default.
- W2891246271 cites W3189225753 @default.
- W2891246271 doi "https://doi.org/10.1016/j.ab.2018.09.010" @default.
- W2891246271 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30218639" @default.
- W2891246271 hasPublicationYear "2018" @default.
- W2891246271 type Work @default.
- W2891246271 sameAs 2891246271 @default.
- W2891246271 citedByCount "4" @default.
- W2891246271 countsByYear W28912462712019 @default.
- W2891246271 countsByYear W28912462712020 @default.
- W2891246271 countsByYear W28912462712021 @default.
- W2891246271 crossrefType "journal-article" @default.
- W2891246271 hasAuthorship W2891246271A5000219705 @default.
- W2891246271 hasAuthorship W2891246271A5000391285 @default.
- W2891246271 hasAuthorship W2891246271A5000706755 @default.
- W2891246271 hasAuthorship W2891246271A5002728543 @default.
- W2891246271 hasAuthorship W2891246271A5034131186 @default.
- W2891246271 hasAuthorship W2891246271A5047392255 @default.
- W2891246271 hasAuthorship W2891246271A5061896931 @default.
- W2891246271 hasAuthorship W2891246271A5071361779 @default.
- W2891246271 hasConcept C147483822 @default.
- W2891246271 hasConcept C151730666 @default.
- W2891246271 hasConcept C153911025 @default.
- W2891246271 hasConcept C159654299 @default.
- W2891246271 hasConcept C179247698 @default.
- W2891246271 hasConcept C181199279 @default.
- W2891246271 hasConcept C185592680 @default.
- W2891246271 hasConcept C195616568 @default.
- W2891246271 hasConcept C203014093 @default.
- W2891246271 hasConcept C25824698 @default.
- W2891246271 hasConcept C2776194381 @default.
- W2891246271 hasConcept C2776600866 @default.
- W2891246271 hasConcept C2779881004 @default.
- W2891246271 hasConcept C2780758401 @default.
- W2891246271 hasConcept C2780762169 @default.
- W2891246271 hasConcept C43617362 @default.
- W2891246271 hasConcept C55493867 @default.
- W2891246271 hasConcept C86803240 @default.
- W2891246271 hasConceptScore W2891246271C147483822 @default.
- W2891246271 hasConceptScore W2891246271C151730666 @default.
- W2891246271 hasConceptScore W2891246271C153911025 @default.
- W2891246271 hasConceptScore W2891246271C159654299 @default.
- W2891246271 hasConceptScore W2891246271C179247698 @default.