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- W2891250189 abstract "ABSTRACT Transcriptional pausing underpins regulation of cellular RNA biogenesis. A consensus pause sequence that acts on RNA polymerases (RNAPs) from bacteria to mammals halts RNAP in an elemental paused state from which longer-lived pauses can arise. Although the structural foundations of pauses prolonged by backtracking or nascent RNA hairpins are recognized, the fundamental mechanism of the elemental pause is less well-defined. Here we report a mechanistic dissection that establishes the elemental pause signal ( i ) is multipartite; ( ii ) causes a modest conformational shift that puts RNAP in an off-pathway state in which template base loading but not RNA translocation is inhibited; and ( iii ) can easily enter pretranslocated and one-base-pair backtracked states despite principally occupying the half-translocated state observed in cryo-EM structures of paused RNAPs. Our findings provide a mechanistic basis for the elemental pause and a framework to understand how pausing is modulated by sequence, cellular conditions, and regulators." @default.
- W2891250189 created "2018-09-27" @default.
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- W2891250189 date "2018-09-19" @default.
- W2891250189 modified "2023-10-18" @default.
- W2891250189 title "The elemental mechanism of transcriptional pausing" @default.
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- W2891250189 doi "https://doi.org/10.1101/422220" @default.
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